CD3D-CD3E Heterodimer 蛋白, Human (Biotinylated, HEK293, Fc-Avi)

CD3D Protein, an integral part of the TCR-CD3 complex on T-lymphocytes, plays a pivotal role in adaptive immunity. It transmits signals through CD3D, CD3E, CD3G, and CD3Z chains upon TCR engagement. CD3D is essential for thymocyte differentiation, ensuring proper TCR-CD3 complex assembly and surface expression. Interacting with CD4 and CD8 coreceptors, CD3D establishes a functional link crucial for CD4 or CD8 T-cell activation and positive selection. Its multifaceted role orchestrates intricate T-cell responses. CD3D-CD3E Heterodimer Protein, Human (Biotinylated, HEK293, Fc-Avi) is a recombinant protein dimer complex containing human-derived CD3D-CD3E Heterodimer protein, expressed by HEK293, with C-Avi, C-hFc labeled tag. CD3D-CD3E Heterodimer Protein, Human (Biotinylated, HEK293, Fc-Avi), has molecular weight of 48-60 kDa.

CD3D 蛋白是 T 淋巴细胞表面 TCR-CD3 复合物的组成部分，在适应性免疫反应中发挥着关键作用。T 细胞受体 (TCR) 被抗原呈递细胞 (APC) 激活，通过 CD3 链 CD3D、CD3E、CD3G 和 CD3Z 传递信号，这些链的胞质结构域中包含基于免疫受体酪氨酸的激活基序 (ITAM)。TCR 参与后，这些 ITAM 被 Src 家族蛋白酪氨酸激酶 LCK 和 FYN 磷酸化，激活下游信号通路。除了在 T 细胞激活的信号转导中发挥作用外，CD3D 对于胸腺细胞分化也是不可或缺的，有助于 TCR-CD3 复合物的正确细胞内组装和表面表达。缺乏功能性 TCR-CD3 复合物的胸腺细胞面临着分化障碍。CD3D 还与辅助受体 CD4 和 CD8 相互作用，在 TCR 和辅助受体之间建立功能联系，这对 CD4 或 CD8 T 细胞的激活和阳性选择至关重要。TCR-CD3复合物包含CD3D/CD3E和CD3G/CD3E异二聚体，形成TCRα/CD3E/CD3G和TCRbeta/CD3G/CD3E三聚体，这些三聚体依次与CD3Z同二聚体相互作用以完成六聚体TCR-CD3复合物。或者，TCRα和TCRβ可以被TCRγ和TCRδ替代。这些复杂的相互作用凸显了 CD3D 在协调 T 细胞反应中的多方面作用。

Measured by its binding ability in a functional ELISA. When immobilized Biotinylated Human CD3E&CD3D, Fc Tag at 1 μg/mL(100 μl/Well) on streptavidin (5 μg/mL) precoated plate, can bind OKT3, mFc Tag and the EC₅₀ is 14.1 ng/mL.

Human

HEK293

C-Avi;C-hFc

P04234 (F22-A105)&P07766 (D23-D126)

915  [NCBI]&916  [NCBI]

48-60 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.