CD40 蛋白, Human (Biotinylated, HEK293, His-Avi)

CD40 protein is a TNFSF5/CD40LG receptor that transduces signals through TRAF6 and MAP3K8-mediated pathways, activates ERK in macrophages and B cells, and induces immunoglobulin secretion. CD40 exists as monomers and homodimers and interacts with TRAF proteins (TRAF1, TRAF2, TRAF3, TRAF5, and TRAF6). CD40 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD40 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of CD40 Protein, Human (Biotinylated, HEK293, His-Avi) is 173 a.a., with molecular weight of 35-40 kDa.

CD40 蛋白作为 TNFSF5/CD40LG 的受体，有助于通过 TRAF6 和 MAP3K8 介导的途径转导信号，从而激活巨噬细胞和 B 细胞中的 ERK，并随后诱导免疫球蛋白分泌。CD40 蛋白以单体和同型二聚体形式存在，其同型二聚体结构表现出变化，如在膀胱癌细胞系 Hu549 中观察到的那样。该受体与 TRAF1、TRAF2、TRAF3、TRAF5 和 TRAF6 等关键信号分子相互作用，其中关键的相互作用发生在 CD40 蛋白、TRAF6 和 MAP3K8 之间，从而在 ERK 激活中发挥关键作用。

Immobilized Biotinylated Human CD40 His at 1 μg/mL (100μL/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) hFc Tag with the EC₅₀ is 33- 87.8 ng/ml determined by ELISA.

Human

HEK293

C-Avi;C-His

P25942 (E21-R193)

958  [NCBI]

35-40 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.