CD45 蛋白, Mouse (HEK293, His)

CD45/PTPRC Protein, Mouse (HEK 293, His) expresses in HEK293 with a His tag at the N-terminus. CD45 is a transmembrane glycoprotein, expressed on almost all haematopoietic cells except for mature erythrocytes. PTPRC is an essential regulator of T and B cell antigen receptor-mediated activation^[1].

CD45 通过其配体巨噬细胞半乳糖型凝集素调节 T 细胞和巨噬细胞之间的相互作用，巨噬细胞半乳糖型凝集素与 CD45 N-乙酰半乳糖胺结合，导致 T 细胞增殖水平降低，产生促炎细胞因子，导致 T 细胞凋亡^{[1]< /sup>。}

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

HEK293

C-6*His

P06800 (Q26-K566)

19264  [NCBI]

QTPTPSDELSTTENALLLPQSDPLPARTTESTPPSISERGNGSSETTYHPGVLSTLLPHLSPQPDSQTPSAGGADTQTFSSQADNPTLTPAPGGGTDPPGVPGERTVPGTIPADTAFPVDTPSLARNSSAASPTHTSNVSTTDISSGASLTTLTPSTLGLASTDPPSTTIATTTKQTCAAMFGNITVNYTYESSNQTFKADLKDVQNAKCGNEDCENVLNNLEECSQIKNISVSNDSCAPATTIDLYVPPGTDKFSLHDCTPKEKANTSICLEWKTKNLDFRKCNSDNISYVLHCEPENNTKCIRRNTFIPERCQLDNLRAQTNYTCVAEILYRGVKLVKNVINVQTDLGIPETPKPSCGDPAARKTLVSWPEPVSKPESASKPHGYVLCYKNNSEKCKSLPNNVTSFEVESLKPYKYYEVSLLAYVNGKIQRNGTAEKCNFHTKADRPDKVNGMKTSRPTDNSINVTCGPPYETNGPKTFYILVVRSGGSFVTKYNKTNCQFYVDNLYYSTDYEFLVSFHNGVYEGDSVIRNESTNFNAK

120-160 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Maryam Ahmed Al Barashdi, et al. Protein tyrosine phosphatase receptor type C (PTPRC or CD45). J Clin Pathol. 2021 Sep;74(9):548-552.  [Content Brief]