Chondroitinase ABC 蛋白, P. vulgaris (His)

Chondroitinase ABC protein is an endolytic glycosaminoglycan lyase with broad specificity that effectively degrades polysaccharides such as chondroitin, chondroitin 4-sulfate, chondroitin 6-sulfate, dermatan sulfate, and smaller degree of hyaluronic acid. This enzyme activity is facilitated by β-elimination of the 1,4-hexosamine bond, resulting in unsaturated tetra- and disaccharides. Chondroitinase ABC Protein, P. vulgaris (His) is the recombinant Chondroitinase ABC protein, expressed by E. coli , with N-His, N-6*His labeled tag. The total length of Chondroitinase ABC Protein, P. vulgaris (His) is 997 a.a., with molecular weight of ~110 kDa.

软骨素酶 ABC 蛋白是一种具有广泛特异性的内溶糖胺聚糖裂解酶，能够降解各种多糖，包括软骨素、4-硫酸软骨素、6-硫酸软骨素、硫酸皮肤素，以及较小程度的透明质酸。该酶通过 1,4-己糖胺键的 β-消除发挥作用，产生不饱和四糖和二糖。重要的是，软骨素酶 ABC 不表现出针对硫酸角质素、硫酸乙酰肝素和肝素的活性。它破坏细胞外基质 (ECM) 正常组织的能力使其成为促进受损中枢神经系统 (CNS) 功能恢复的关键角色。这强调了其通过对 ECM 内的特定糖胺聚糖进行靶向修饰来促进神经再生和修复的潜在治疗意义。（

Measured by its ability to hydrolyze chondroitin sulfate. The specific activity is >15,000 pmol/min/μg.

Others

E. coli

N-His;N-6*His

P59807 (A25-P1021)

2827933  [NCBI]

ATSNPAFDPKNLMQSEIYHFAQNNPLADFSSDKNSILTLSDKRSIMGNQSLLWKWKGGSSFTLHKKLIVPTDKEASKAWGRSSTPVFSFWLYNEKPIDGYLTIDFGEKLISTSEAQAGFKVKLDFTGWRAVGVSLNNDLENREMTLNATNTSSDGTQDSIGRSLGAKVDSIRFKAPSNVSQGEIYIDRIMFSVDDARYQWSDYQVKTRLSEPEIQFHNVKPQLPVTPENLAAIDLIRQRLINEFVGGEKETNLALEENISKLKSDFDALNIHTLANGGTQGRHLITDKQIIIYQPENLNSQDKQLFDNYVILGNYTTLMFNISRAYVLEKDPTQKAQLKQMYLLMTKHLLDQGFVKGSALVTTHHWGYSSRWWYISTLLMSDALKEANLQTQVYDSLLWYSREFKSSFDMKVSADSSDLDYFNTLSRQHLALLLLEPDDQKRINLVNTFSHYITGALTQVPPGGKDGLRPDGTAWRHEGNYPGYSFPAFKNASQLIYLLRDTPFSVGESGWNNLKKAMVSAWIYSNPEVGLPLAGRHPFNSPSLKSVAQGYYWLAMSAKSSPDKTLASIYLAISDKTQNESTAIFGETITPASLPQGFYAFNGGAFGIHRWQDKMVTLKAYNTNVWSSEIYNKDNRYGRYQSHGVAQIVSNGSQLSQGYQQEGWDWNRMQGATTIHLPLKDLDSPKPHTLMQRGERGFSGTSSLEGQYGMMAFDLIYPANLERFDPNFTAKKSVLAADNHLIFIGSNINSSDKNKNVETTLFQHAITPTLNTLWINGQKIENMPYQTTLQQGDWLIDSNGNGYLITQAEKVNVSRQHQVSAENKNRQPTEGNFSSAWIDHSTRPKDASYEYMVFLDATPEKMGEMAQKFRENNGLYQVLRKDKDVHIILDKLSNVTGYAFYQPASIEDKWIKKVNKPAIVMTHRQKDTLIVSAVTPDLNMTRQKAATPVTINVTINGKWQSADKNSEVKYQVSGDNTELTFTSYFGIPQEIKLSPLP

Approximately110 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Liquid from sterile 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.