1. 重组蛋白
  2. Receptor Proteins
  3. CHRNB3 蛋白, Human (HEK293, His)

CHRNB3 蛋白, Human (HEK293, His)

目录号: HY-P70056
产品使用指南

CHRNB3 蛋白是乙酰胆碱受体 (AChR) 的重要组成部分,在乙酰胆碱结合时诱导显着的构象转变。这种变化影响所有亚基,导致跨质膜的离子传导通道激活。神经元 AChR 复合物由 α 和 β 亚基组成,强调它们在响应乙酰胆碱刺激方面的复杂协作。CHRNB3 蛋白, Human (HEK293, His) 是重组的 CHRNB3 蛋白,由 HEK293 表达,带有 C-6*His 标签。CHRNB3 蛋白, Human (HEK293, His) 全长 208 个氨基酸,分子量为 30-40 kDa。

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描述

CHRNB3 Protein, a vital constituent of the acetylcholine receptor (AChR), induces a significant conformational shift upon acetylcholine binding. This change affects all subunits, leading to the activation of an ion-conducting channel across the plasma membrane. The neuronal AChR complex comprises alpha and beta subunits, emphasizing their intricate collaboration in responding to acetylcholine stimulation. CHRNB3 Protein, Human (HEK293, His) is the recombinant human-derived CHRNB3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CHRNB3 Protein, Human (HEK293, His) is 208 a.a., with molecular weight of 30-40 kDa.

研究背景

乙酰胆碱受体 (AChR) 的重要组成部分 CHRNB3 与乙酰胆碱结合后,会引发影响所有亚基的深刻构象变化,最终导致跨质膜的离子传导通道打开。神经元 AChR 复合物被认为由两种不同类型的亚基组成,即 α 和 β,突出显示这些亚基之间在介导细胞对乙酰胆碱刺激的反应中复杂的相互作用。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q05901 (I25-L232)

基因 ID
蛋白结构
N-term
CHRNB3 (I25-L232)
Accession # Q05901
6*His
C-term
同用名
rHuNeuronal acetylcholine receptor subunit beta-3/CHRNB3, His ; Neuronal acetylcholine receptor subunit beta-3
氨基酸序列

IAENEDALLRHLFQGYQKWVRPVLHSNDTIKVYFGLKISQLVDVDEKNQLMTTNVWLKQEWTDHKLRWNPDDYGGIHSIKVPSESLWLPDIVLFENADGRFEGSLMTKVIVKSNGTVVWTPPASYKSSCTMDVTFFPFDRQNCSMKFGSWTYDGTMVDLILINENVDRKDFFDNGEWEILNAKGMKGNRRDGVYSYPFITYSFVLRRL

分子量

30-40 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CHRNB3 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CHRNB3 Protein, Human (HEK293, His)
目录号:
HY-P70056
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