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  4. COL21A1 蛋白, Human (HEK293, His)

COL21A1 蛋白, Human (HEK293, His)

目录号: HY-P70094
产品使用指南

COL21A1 蛋白是纤维相关胶原中断螺旋 (FACIT) 家族的成员。COL21A1 蛋白是一种分泌蛋白广泛存在于多种组织中。COL21A1 蛋白 在血管形成过程中可能有助于血管网络的细胞外基质组装。COL21A1 蛋白, Human (HEK293, His) 是重组的 COL21A1 蛋白，由 HEK293 表达，带有 C-6*His 标签。COL21A1 蛋白, Human (HEK293, His) 全长 935 个氨基酸，分子量分别为 115-125 & 220 kDa。

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100 μg   询价  

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描述

COL21A1 Protein is a member of the fibre-associated collagen interrupt helix (FACIT) family. COL21A1 Protein is a secreted protein widely present in a variety of tissues. COL21A1 Protein may contribute to the extracellular matrix assembly of vascular networks during angiogenesis. COL21A1 Protein, Human (HEK293, His) is the recombinant human-derived COL21A1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of COL21A1 Protein, Human (HEK293, His) is 935 a.a., with molecular weight of 115-125 & 220 kDa, respectively.
研究背景

Collagen alpha-1 (XXI) chain 是一种在人类中由 COL21A1 基因编码的蛋白质，是 FACIT (纤维相关胶原蛋白中断螺旋) 胶原蛋白家族的一员。COL21A1 在人体组织中的表达受发育调控，在胎儿阶段表达水平较高。XXI 型胶原是血管壁的细胞外基质成分，由平滑肌细胞分泌。血小板衍生生长因子 (PDGF) 对体外培养的主动脉平滑肌细胞 COL21A1 表达的刺激有显著作用，提示在血管形成过程中，alpha1 (XXI) 胶原可能有助于血管网络的细胞外基质组装。
种属

Human
表达系统

HEK293
标签

C-6*His
蛋白编号

Q96P44 (E23-Y957)
基因 ID
蛋白结构
N-term
COL21A1 (E23-Y957)
Accession # Q96P44
6*His
C-term
同用名
rHuCollagen alpha-1(XXI) chain/COL21A1, His ; COL21A1; collagen, type XXI, alpha 1; collagen alpha-1(XXI) chain; alpha 1 type XXI collagen; FP633; COLA1L; dJ708F5.1; dJ682J15.1; FLJ39125; FLJ44623; MGC26619; DKFZp564B052
氨基酸序列

EDGEVRSSCRTAPTDLVFILDGSYSVGPENFEIVKKWLVNITKNFDIGPKFIQVGVVQYSDYPVLEIPLGSYDSGEHLTAAVESILYLGGNTKTGKAIQFALDYLFAKSSRFLTKIAVVLTDGKSQDDVKDAAQAARDSKITLFAIGVGSETEDAELRAIANKPSSTYVFYVEDYIAISKIREVMKQKLCEESVCPTRIPVAARDERGFDILLGLDVNKKVKKRIQLSPKKIKGYEVTSKVDLSELTSNVFPEGLPPSYVFVSTQRFKVKKIWDLWRILTIDGRPQIAVTLNGVDKILLFTTTSVINGSQVVTFANPQVKTLFDEGWHQIRLLVTEQDVTLYIDDQQIENKPLHPVLGILINGQTQIGKYSGKEETVQFDVQKLRIYCDPEQNNRETACEIPGFNGECLNGPSDVGSTPAPCICPPGKPGLQGPKGDPGLPGNPGYPGQPGQDGKPGYQGIAGTPGVPGSPGIQGARGLPGYKGEPGRDGDKGDRGLPGFPGLHGMPGSKGEMGAKGDKGSPGFYGKKGAKGEKGNAGFPGLPGPAGEPGRHGKDGLMGSPGFKGEAGSPGAPGQDGTRGEPGIPGFPGNRGLMGQKGEIGPPGQQGKKGAPGMPGLMGSNGSPGQPGTPGSKGSKGEPGIQGMPGASGLKGEPGATGSPGEPGYMGLPGIQGKKGDKGNQGEKGIQGQKGENGRQGIPGQQGIQGHHGAKGERGEKGEPGVRGAIGSKGESGVDGLMGPAGPKGQPGDPGPQGPPGLDGKPGREFSEQFIRQVCTDVIRAQLPVLLQSGRIRNCDHCLSQHGSPGIPGPPGPIGPEGPRGLPGLPGRDGVPGLVGVPGRPGVRGLKGLPGRNGEKGSQGFGYPGEQGPPGPPGPEGPPGISKEGPPGDPGLPGKDGDHGKPGIQGQPGPPGICDPSLCFSVIARRDPFRKGPNY

分子量

115-125&220 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

COL21A1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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