COL4A2 蛋白, Human (His)

COL4A2 protein is a key type IV collagen element that forms the main structural component of the glomerular basement membrane (GBM), forming a unique "chicken wire" network structure with laminin, proteoglycans and nestin/nesidin. . Canstatin is a cleavage product of the NC1 domain of collagen α 2(IV) and has potent anti-angiogenic and anti-tumor activities. COL4A2 Protein, Human (His) is the recombinant human-derived COL4A2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of COL4A2 Protein, Human (His) is 220 a.a., with molecular weight of ~28.3 kDa.

COL4A2 蛋白是 IV 型胶原蛋白的基本成分，作为肾小球基底膜 (GBM) 的主要结构成分发挥着核心作用，与层粘连蛋白、蛋白聚糖和巢蛋白/巢蛋白一起形成特征性的“鸡丝”网络。在此背景下，Canstatin（一种源自胶原蛋白 α 2(IV) NC1 结构域的裂解产物）表现出显着的抗血管生成和抗肿瘤细胞活性。Canstatin 干扰内皮细胞的增殖和迁移，降低线粒体膜电位，并诱导细胞凋亡。其机制包括特异性诱导 Fas 依赖性细胞凋亡，以及激活 procaspase-8 和 -9 活性。值得注意的是，Canstatin 作为 alphavbeta3 和 alphavbeta5 整合素的配体，强调了其在 GBM 等富含 IV 型胶原蛋白的复杂环境中调节细胞反应的多方面作用。

Human

E. coli

N-6*His

P08572 (V1493-L1712)

1284  [NCBI]

VKHSQTDQEPMCPVGMNKLWSGYSLLYFEGQEKAHNQDLGLAGSCLARFSTMPFLYCNPGDVCYYASRNDKSYWLSTTAPLPMMPVAEDEIKPYISRCSVCEAPAIAIAVHSQDVSIPHCPAGWRSLWIGYSFLMHTAAGDEGGGQSLVSPGSCLEDFRATPFIECNGGRGTCHYYANKYSFWLTTIPEQSFQGSPSADTLKAGLIRTHISRCQVCMKNL

Approximately 28.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.