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  3. COL4A3 蛋白, Rat (HEK293, Fc)

COL4A3 蛋白, Rat (HEK293, Fc)

目录号: HY-P75683
COA 产品使用指南

COL4A3 蛋白是一种关键的 IV 型胶原蛋白成分,形成肾小球基底膜 (GBM) 的主要结构元素。它与层粘连蛋白、蛋白聚糖和巢蛋白/巢蛋白合作创建一个“鸡丝”网络,这对于维持 GBM 的结构完整性至关重要。COL4A3 蛋白, Rat (HEK293, Fc) 是重组的 COL4A3 蛋白,由 HEK293 表达,带有 N-hFc 标签。COL4A3 蛋白, Rat (HEK293, Fc) 全长 245 个氨基酸,分子量约为 ~59 kDa。

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描述

The COL4A3 protein is a key type IV collagen component that forms the major structural element of the glomerular basement membrane (GBM). It collaborates with laminin, proteoglycans, and nestin/nesidin to create a "chicken wire" network that is critical for maintaining the structural integrity of the GBM. COL4A3 Protein, Rat (HEK293, Fc) is the recombinant rat-derived COL4A3 protein, expressed by HEK293 , with N-hFc labeled tag. The total length of COL4A3 Protein, Rat (HEK293, Fc) is 245 a.a., with molecular weight of ~59 kDa.

研究背景

COL4A3 蛋白是 IV 型胶原蛋白的重要组成部分,是肾小球基底膜 (GBM) 的主要结构元件,它与层粘连蛋白、蛋白聚糖和巢蛋白/巢蛋白合作创建“鸡丝”网络。这种复杂的网络在维持 GBM 的结构完整性和稳定性方面发挥着基础作用,这对于它们在肾脏生理学中的正常功能至关重要。COL4A3 参与网状结构的形成强调了其在支持肾小球基底膜完整性方面的重要性,肾小球基底膜对于肾功能和体内平衡至关重要。

种属

Rat

表达系统

HEK293

标签

N-hFc

蛋白编号

F1LRJ1 (G1426-H1670)

基因 ID

363265  [NCBI]

蛋白结构
N-term
hFc
COL4A3 (G1426-H1670)
Accession # F1LRJ1
C-term
同用名
Collagen alpha-3(IV) chain; Goodpasture antigen; Tumstatin; COL4A3
分子量

Approximately 59 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

COL4A3 Protein, Rat (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
COL4A3 Protein, Rat (HEK293, Fc)
目录号:
HY-P75683
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