Collagen alpha-1(II) chain/COL2A1 蛋白, Human (HEK293, His)

COL2A1, encoding the alpha-1(II) chain of type II collagen, is specific to cartilaginous tissues, essential for embryonic skeletal development, linear growth, and cartilage resilience against compressive forces. As a fundamental component of homotrimers, COL2A1 maintains the structural integrity and function of cartilage, crucial for skeletal form and function during development and in resisting mechanical stress. Collagen alpha-1 (II) chain/COL2A1 Protein, Human (HEK293, His) is the recombinant human-derived Collagen alpha-1(II) chain/COL2A1 protein, expressed by HEK293 , with N-His labeled tag. The total length of Collagen alpha-1 (II) chain/COL2A1 Protein, Human (HEK293, His) is 246 a.a., with molecular weight of ~32-37 kDa.

COL2A1 蛋白编码 II 型胶原蛋白的 α-1(II) 链，对软骨组织具有特异性，在正常胚胎骨骼发育、线性生长和软骨抵抗压力的弹性中发挥着至关重要的作用。作为同源三聚体的基本组成部分，COL2A1 有助于软骨的结构完整性和功能，突显其在整个发育过程中维持骨骼形态和功能以及抵抗机械应力方面不可或缺的作用。

Measured by its binding ability in a functional ELISA. When Recombinant Human COL2A1 is present at 2 μg/mL, can bind anti-COL2A1 Antibody.The ED50 for this effect is 188.4 ng/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Human COL2A1 is present at 2 μg/mL, can bind anti-COL2A1 Antibody.The ED₅₀ for this effect is 188.4 ng/mL.

Human

HEK293

N-His

P02458-2 (D1242-L1487)

1280  [NCBI]

DQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNPANVPKKNWWSSKSKEKKHIWFGETINGGFHFSYGDDNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSNDVEIRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPMDIGGPEQEFGVDIGPVCFL

Approximately 32-37 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.