CTCF 蛋白, Human

CTCF protein is a chromatin-binding factor that plays multiple roles in transcriptional regulation and epigenetic control. It binds to DNA at specific sites and acts as a transcriptional repressor by binding to chromatin insulators to prevent undesirable interactions between promoters and neighboring enhancers or silencers. CTCF Protein, Human is the recombinant human-derived CTCF protein, expressed by E. coli , with tag free. The total length of CTCF Protein, Human is 154 a.a., with molecular weight of ~38 kDa.

CTCF 蛋白是一种染色质结合因子，在转录调控和表观遗传控制中发挥多种作用。它与 DNA 序列特异性位点结合，通过与染色质绝缘子相互作用而充当转录抑制子，防止启动子与附近的增强子或沉默子之间发生不需要的相互作用。值得注意的是，它抑制 MYC 和 BAG1 等基因的启动子，同时激活 APP 的转录。CTCF调节APOA1/C3/A4/A5基因簇，控制MHC II类基因表达，在卵母细胞和植入前胚胎发育中发挥至关重要的作用。作为肿瘤抑制因子，CTCF 参与印记 IGF2/H19 基因座的等位基因特异性基因表达，并参与相当大的基因组距离上的基因沉默。它优先与未甲基化的 DNA 相互作用，防止 CpG 甲基化扩散并维持无甲基化区域。CTCF 对于染色质重塑至关重要，当与不同的 DNA 序列结合以介导长程染色质环化时会形成二聚体。它锚定核小体，影响 X 染色体配对，并有助于 X 染色体失活的可调节表观遗传开关。此外，CTCF 在中期与着丝粒和染色体臂结合，有助于姐妹染色单体的凝聚力，并在有丝分裂期间将 CENPE 招募到着丝粒周围/着丝粒区域。它与 CHD8 和 LLPH 的相互作用及其多方面的调节功能，强调了 CTCF 在复杂的染色质组织和基因表达中的关键作用。

Human

E. coli

Tag Free

P49711 (M1-I154)

10664  [NCBI]

MEGDAVEAIVEESETFIKGKERKTYQRRREGGQEEDACHLPQNQTDGGEVVQDVNSSVQMVMMEQLDPTLLQMKTEVMEGTVAPEAEAAVDDTQIITLQVVNMEEQPINIGELQLVQVPVPVTVPVATTSVEELQGAYENEVSKEGLAESEPMI

Approximately 38 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.