CXCL8 蛋白, Rhesus macaque (His)

CXCL8 protein, a crucial chemotactic factor, drives inflammatory responses by attracting neutrophils, basophils, and T-cells, contributing to pathogen clearance. It plays a pivotal role in activating neutrophils and binds to CXCR1/CXCR2 receptors, initiating downstream signaling pathways. CXCL8 homodimerizes and interacts with TNFAIP6, potentially regulating chemokine activity in the inflammatory microenvironment. CXCL8 Protein, Rhesus macaque (His) is the recombinant Rhesus Macaque-derived CXCL8 protein, expressed by E. coli , with N-6*His labeled tag. The total length of CXCL8 Protein, Rhesus macaque (His) is 79 a.a., with molecular weight of ~11 kDa.

CXCL8 蛋白作为关键的趋化因子，通过吸引中性粒细胞、嗜碱性粒细胞和 T 细胞来协调炎症反应，从而帮助清除病原体并保护宿主免受感染。此外，CXCL8 在激活中性粒细胞中发挥着关键作用。CXCL8 因炎症刺激而释放后，通过与 G 蛋白偶联受体 CXCR1 和 CXCR2（主要存在于中性粒细胞、单核细胞和内皮细胞中）结合来发挥作用。G 蛋白异三聚体（α、β、γ 亚基）与 CXCR1/CXCR2 受体组成型结合，CXCL8 激活导致 Gα（中性粒细胞中的 GNAI2）释放 β 和 γ 亚基，随后激活各种下游信号通路，包括 PI3K 和 MAPK 通路。CXCL8 形成同型二聚体，并且其通过 Link 结构域与 TNFAIP6 相互作用，干扰趋化因子与糖胺聚糖的结合，表明在调节炎症微环境中趋化因子活性方面具有调节作用。

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells.The ED₅₀ for this effect is 8.054-11.39 ng/mL, corresponding to a specific activity is 1.24×10⁵ to 8.78×10⁴ units/mg.

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  Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells.The ED₅₀ for this effect is 8.054-11.39 ng/mL, corresponding to a specific activity is 1.24×10⁵ to 8.78×10⁴ units/mg.

Rhesus Macaque

E. coli

N-6*His

P67813 (A23-P101)

613028  [NCBI]

AVLPRSAKELRCECIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKEPWVQRVVEKFVKRAENQNP

Approximately 11 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.