2. 重组蛋白
  3. Enzymes & Regulators
  4. Protease Inhibitors Cystatin Family
  5. Cystatin B
  6. Cystatin B/CSTB 蛋白, Mouse (His)

Cystatin B/CSTB 蛋白, Mouse (His)

目录号: HY-P70137
产品使用指南

胱抑素 B/CSTB 蛋白是一种细胞内硫醇蛋白酶抑制剂，对细胞内的蛋白水解活性具有重要调节作用。通过抑制硫醇蛋白酶，半胱氨酸蛋白酶抑制剂 B 维持蛋白水解过程的平衡，强调其在细胞稳态中的重要性。其抑制功能强调参与需要精确调节蛋白水解活性以维持正常细胞功能和完整性的细胞过程。Cystatin B/CSTB 蛋白, Mouse (His) 是重组的 胱抑素 B/CSTB 蛋白，由 E. coli 表达，带有 N-6*His 标签。Cystatin B/CSTB 蛋白, Mouse (His) 全长 98 个氨基酸，分子量约为 ~15.0 kDa。

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描述

Cystatin B/CSTB Protein, an intracellular thiol proteinase inhibitor, crucially regulates proteolytic activities within the cell. By inhibiting thiol proteinases, Cystatin B maintains the balance of proteolytic processes, emphasizing its significance in cellular homeostasis. Its inhibitory function underscores involvement in cellular processes requiring precise regulation of proteolytic activity for normal cellular function and integrity. Cystatin B/CSTB Protein, Mouse (His) is the recombinant mouse-derived Cystatin B/CSTB protein, expressed by E. coli , with N-6*His labeled tag. The total length of Cystatin B/CSTB Protein, Mouse (His) is 98 a.a., with molecular weight of ~15.0 kDa.
研究背景

Cystatin B/CSTB 蛋白作为细胞内硫醇蛋白酶抑制剂发挥作用。该蛋白具有抑制硫醇蛋白酶的作用，有助于调节细胞内蛋白水解活性。通过调节硫醇蛋白酶的活性，半胱氨酸蛋白酶抑制剂 B 在维持细胞内蛋白水解过程的平衡中发挥着至关重要的作用，凸显了其在细胞稳态中的重要性。半胱氨酸蛋白酶抑制剂 B 对硫醇蛋白酶的抑制功能强调了它参与细胞过程，其中蛋白水解活性的精确调节对于正常细胞功能和完整性至关重要。
种属

Mouse
表达系统

E. coli
标签

N-6*His
蛋白编号

Q62426 (M1-F98)
基因 ID

13014  [NCBI]
蛋白结构
N-term
6*His
CSTB (M1-F98)
Accession # Q62426
C-term
同用名
rMuCystatin-B, His; CPI-B; CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B; CSTB; cystatin B (stefinB); Cystatin B; EPM1; Liver thiol proteinase inhibitor; PME; Stefin B; stefin-B
氨基酸序列

MMCGAPSATMPATAETQEVADQVKSQLESKENQKFDVFKAISFKRQIVAGTNLFIKVDVGGDKCVHLRVFQPLPHENKPLTLSSYQTNKERHDELSYF

分子量

Approximately 15.0 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Cystatin B/CSTB Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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