Cytosolic beta-Glucosidase/GBA3 蛋白, Human (GST)

Cytosolic β-glucosidase/GBA3 is a multifunctional neutral cytosolic β-glucosidase that displays broad substrate specificity, suggesting a possible involvement in glycosylceramide catabolism. Although it exhibits significant glucosylceramidase activity in vitro, its in vivo relevance is unclear. Cytosolic beta-Glucosidase/GBA3 Protein, Human (GST) is the recombinant human-derived Cytosolic beta-Glucosidase/GBA3 protein, expressed by E. coli , with N-GST labeled tag. The total length of Cytosolic beta-Glucosidase/GBA3 Protein, Human (GST) is 162 a.a., with molecular weight of ~45.3 kDa.

胞质 β-葡萄糖苷酶/GBA3 是一种中性胞质 β-糖苷酶，具有广泛的底物特异性，表明可能参与糖基神经酰胺的分解代谢。虽然在体外表现出显着的葡萄糖神经酰胺酶活性，但其体内相关性仍不清楚。该酶还水解半乳糖神经酰胺/GalCers、葡萄糖基鞘氨醇/GlcSphs 和半乳糖基鞘氨醇/GalSphs，尽管这些活性的生理重要性尚未完全阐明。此外，胞质 β-葡萄糖苷酶/GBA3 利用木糖基化神经酰胺/XylCers 作为供体，胆固醇作为受体，在体外显示出转木糖基酶活性。值得注意的是，该酶在水解各种膳食糖苷（包括植物雌激素、黄酮醇、黄酮、黄烷酮和氰）方面表现出多功能性，表明其在异生物质代谢中具有潜在作用。此外，它可能有助于胞质唾液酸游离 N-聚糖的分解代谢。

Human

E. coli

N-GST

Q9H227 (1M-162L)

57733  [NCBI]

MAFPAGFGWAAATAAYQVEGGWDADGKGPCVWDTFTHQGGERVFKNQTGDVACGSYTLWEEDLKCIKQLGLTHYRFSLSWSRLLPDGTTGFINQKAIQLDKVNLQVYCAWSLLDNFEWNQGYSSRFGLFHVDFEDPARPRVPYTSAKEYAKIIRNNGLEAHL

Approximately 45.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.