1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. DBT 蛋白, Human (sf9, His)

DBT 蛋白, Human (sf9, His)

目录号: HY-P74207
产品使用指南

DBT 蛋白是支链 α-酮脱氢酶复合物的组成部分,可催化 α-酮酸转化为酰基辅酶 A 和 CO(2)。该复合物包括支链 α-酮酸脱羧酶 (E1)、硫辛酰胺酰基转移酶 (E2) 和硫辛酰胺脱氢酶 (E3)。DBT 蛋白, Human (sf9, His) 是重组的 DBT 蛋白,由 Sf9 insect cells 表达,带有 N-His 标签。DBT 蛋白, Human (sf9, His) 全长 421 个氨基酸,分子量约为 ~48.8 kDa。

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1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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描述

DBT proteins are components of the branched-chain α-keto dehydrogenase complex, which catalyzes the conversion of α-keto acids into acyl-CoA and CO(2). This complex includes branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2), and lipoamide dehydrogenase (E3). DBT Protein, Human (sf9, His) is the recombinant human-derived DBT protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of DBT Protein, Human (sf9, His) is 421 a.a., with molecular weight of ~48.8 kDa.

研究背景

DBT蛋白是支链α-酮脱氢酶复合物的组成部分,在催化α-酮酸全面转化为酰基辅酶A和CO(2)方面发挥着关键作用。该复合物包含三种不同酶组分的多个拷贝:支链 α-酮酸脱羧酶 (E1)、硫辛酰胺酰基转移酶 (E2) 和硫辛酰胺脱氢酶 (E3)。具体来说,在这个复杂的组装过程中,DBT 酶的催化功能涉及接受并随后将支链 α-酮酸脱羧酶组分产生的酰基转移至辅酶 A。这种酶级联强调了 DBT 在介导代谢途径关键步骤中的重要贡献,促进 α-酮酸转化为生物相关的酰基辅酶 A 分子。

种属

Human

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

P11182 (G62-K482)

基因 ID
蛋白结构
N-term
His
DBT (G62-K482)
Accession # P11182
C-term
同用名
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; BCKAD-E2; DBT
分子量

Approximately 48.8 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

DBT Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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