EPHX2 蛋白, Human (sf9, His)

EPHX2 Protein, a bifunctional enzyme, displays epoxide hydrolase activity in its C-terminal domain, crucial for xenobiotic metabolism. It degrades toxic epoxides and influences steady-state levels of physiological mediators. The N-terminal domain exhibits lipid phosphatase activity, prioritizing threo-9,10-phosphonooxy-hydroxy-octadecanoic acid. EPHX2's multifaceted enzymatic activities underscore its diverse roles in cellular processes, including xenobiotic metabolism and lipid homeostasis. EPHX2 Protein, Human (sf9, His) is the recombinant human-derived EPHX2 protein, expressed by Sf9 insect cells, with N-His labeled tag. The total length of EPHX2 Protein, Human (sf9, His) is 555 a.a., with molecular weight of ~65 kDa.

EPHX2 蛋白是一种双功能酶，其 C 端和 N 端结构域具有不同的活性。C 末端结构域表现出环氧化物水解酶活性，作用于烯烃氧化物和芳烃氧化物。这种功能通过降解潜在有毒的环氧化物，在外源代谢中发挥着至关重要的作用，并有助于确定生理介质的稳态水平。另一方面，EPHX2的N端结构域具有脂质磷酸酶活性，对苏型9,10-膦酰氧基-羟基-十八烷酸表现出最高的活性，其次是赤型-9,10-膦酰氧基-羟基-十八烷酸，12-膦酰氧基-十八-9Z-烯酸和12-膦酰氧基-十八-9E-烯酸。此外，它对溶血甘油磷脂表现出磷酸酶活性，对鞘脂和类异戊二烯磷酸盐的溶血脂具有较低的活性。这些多方面的酶活性凸显了 EPHX2 在细胞过程中的多种作用，包括外源代谢和脂质稳态。

Human

Sf9 insect cells

N-His

P34913 (M1-M555)

2053  [NCBI]

Approximately 65 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.