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  3. ERLIN1 蛋白, Human (Cell-Free, His)

ERLIN1 蛋白, Human (Cell-Free, His)

目录号: HY-P702272
产品使用指南

ERLIN1 蛋白是 ERLIN1/ERLIN2 复合物的重要组成部分,在介导肌醇 1,4,5-三磷酸受体 (IP3R) 的内质网相关降解 (ERAD) 中发挥关键作用。除了参与ERAD之外,ERLIN1还通过调节SREBP信号通路来调节细胞胆固醇稳态。ERLIN1 蛋白, Human (Cell-Free, His) 是重组的 ERLIN1 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。ERLIN1 蛋白, Human (Cell-Free, His) 全长 348 个氨基酸,分子量为 42.0 kDa。

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描述

The ERLIN1 protein is an important component of the ERLIN1/ERLIN2 complex and plays a key role in mediating endoplasmic reticulum-associated degradation (ERAD) of the inositol 1,4,5-trisphosphate receptor (IP3R). In addition to participating in ERAD, ERLIN1 also regulates cellular cholesterol homeostasis by regulating the SREBP signaling pathway. ERLIN1 Protein, Human (Cell-Free, His) is the recombinant human-derived ERLIN1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of ERLIN1 Protein, Human (Cell-Free, His) is 348 a.a., with molecular weight of 42.0 kDa.

研究背景

ERLIN1 是 ERLIN1/ERLIN2 复合物的重要组成部分,促进肌醇 1,4,5-三磷酸受体 (IP3R) 的内质网相关降解 (ERAD)。它在细胞胆固醇稳态调节中的作用值得注意,因为它调节 SREBP 信号通路。ERLIN1 还表现出结合胆固醇的能力,可能促进 SCAP-SREBF 复合物的内质网 (ER) 保留,从而影响胆固醇相关的细胞过程。在微生物感染的背景下,ERLIN1 在丙型肝炎病毒 (HCV) 感染中发挥双重作用,早期需要启动 RNA 复制,后来需要支持感染性病毒颗粒的产生。这种多方面的功能强调了 ERLIN1 在与蛋白质降解、胆固醇调节和病毒感染相关的细胞过程中的重要性。

种属

Human

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

O75477 (M1-G348)

基因 ID

10613

蛋白结构
N-term
10*His
ERLIN1 (M1-G348)
Accession # O75477
C-term
同用名
Erlin-1; Endoplasmic reticulum lipid raft-associated protein 1; Protein KE04; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 1; SPFH domain-containing protein 1
氨基酸序列

MNMTQARVLVAAVVGLVAVLLYASIHKIEEGHLAVYYRGGALLTSPSGPGYHIMLPFITTFRSVQTTLQTDEVKNVPCGTSGGVMIYIDRIEVVNMLAPYAVFDIVRNYTADYDKTLIFNKIHHELNQFCSAHTLQEVYIELFDQIDENLKQALQKDLNLMAPGLTIQAVRVTKPKIPEAIRRNFELMEAEKTKLLIAAQKQKVVEKEAETERKKAVIEAEKIAQVAKIRFQQKVMEKETEKRISEIEDAAFLAREKAKADAEYYAAHKYATSNKHKLTPEYLELKKYQAIASNSKIYFGSNIPNMFVDSSCALKYSDIRTGRESSLPSKEALEPSGENVIQNKESTG

分子量

42.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ERLIN1 Protein, Human (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ERLIN1 Protein, Human (Cell-Free, His)
目录号:
HY-P702272
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