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  3. ESRP1/RBM35A 蛋白, Human (sf9, His)

ESRP1/RBM35A 蛋白, Human (sf9, His)

目录号: HY-P77362
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ESRP1/RBM35A 蛋白是一种重要的 mRNA 剪接因子,尤其影响上皮细胞特异性亚型,例如 FGFR2-IIIb。在上皮间质转化 (EMT) 过程中,其调节影响延伸至转录本,包括 CD44、CTNND1 和 ENAH。ESRP1/RBM35A 蛋白, Human (sf9, His) 是重组的 ESRP1/RBM35A 蛋白,由 Sf9 insect cells 表达,带有 N-His 标签。ESRP1/RBM35A 蛋白, Human (sf9, His) 全长 681 个氨基酸,分子量约为 ~77.9 KDa。

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描述

The ESRP1/RBM35A protein is an important mRNA splicing factor, particularly affecting epithelial cell-specific isoforms such as FGFR2-IIIb. During epithelial-mesenchymal transition (EMT), its regulatory influence extends to transcripts including CD44, CTNND1, and ENAH. ESRP1/RBM35A Protein, Human (sf9, His) is the recombinant human-derived ESRP1/RBM35A protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of ESRP1/RBM35A Protein, Human (sf9, His) is 681 a.a., with molecular weight of ~77.9 KDa.

研究背景

ESRP1/RBM35A 蛋白作为重要的 mRNA 剪接因子,复杂地调节上皮细胞特异性亚型的生成。其靶向影响在 FGFR2-IIIb(FGFR2 的上皮细胞特异性亚型)的调节中尤为明显。此外,ESRP1/RBM35A 将其调控范围扩展到关键转录物 CD44、CTNND1 和 ENAH,这些转录物在上皮间质转化 (EMT) 过程中经历剪接改变。它通过直接结合特定 mRNA 序列发挥作用,特异性靶向 ISE/ISS-3(FGFR2 mRNA 内的顺式元件调控区域)中富含 GU 的序列基序。这种对剪接的微妙控制超出了上皮-间质转化的范围,因为 ESRP1/RBM35A 还控制着对内耳发育、听毛细胞分化和耳蜗上皮细胞命运规范至关重要的基因的剪接和表达。

种属

Human

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

Q6NXG1 (M1-I681)

基因 ID
蛋白结构
N-term
His
ESRP1 (M1-I681)
Accession # Q6NXG1
C-term
同用名
Epithelial splicing regulatory protein 1; RNA-binding protein 35A; RBM35A
分子量

Approximately 77.9 kDa.

纯度

Greater than 80% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ESRP1/RBM35A Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ESRP1/RBM35A Protein, Human (sf9, His)
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HY-P77362
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