FAP 蛋白, Cynomolgus (HEK293, His)

FAP Protein is a type II transmembrane serine protease, a member of the propionyl peptidase family, that promotes cell invasion and tumor growth. FAP protein has the function of enhancing blood coagulation. FAP Protein is associated with a variety of human pathology, including fibrosis, arthritis, atherosclerosis, autoimmune diseases, metabolic diseases and cancer. FAP Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived FAP protein, expressed by HEK293 , with N-His labeled tag. The total length of FAP Protein, Cynomolgus (HEK293, His) is 731 a.a., with molecular weight of ~87 kDa.

FAP 蛋白是一种 II 型跨膜丝氨酸蛋白酶，可促进细胞侵袭性和肿瘤生长，几乎完全表达于纤维化、关节炎和癌症等病理状况。FAP Protein具有二肽基肽酶活性，从而能够裂解神经肽 Y、肽 YY、P 物质和脑利钠肽。FAP Protein 能切割 α-2 抗纤溶酶，可将α2-抗纤溶酶转化为更有效的纤溶酶抑制剂，具有增强凝血的功能。FAP Protein 通过促进增殖、侵袭、血管生成、上皮间充质转化、干细胞促进、免疫抑制和耐药性等多个机制来促进肿瘤生长。FAP Protein 与多种人类病理有关，包括纤维化、关节炎、动脉粥样硬化、自身免疫性疾病、代谢疾病和癌症^[1][2]。
将FAP Protein 靶向癌症相关成纤维细胞可以实现实体瘤的高度特异性成像和治疗^[3]。

Measured by its ability to convert the substrate benzyloxycarbonyl­Gly­Pro­7­amido­4­methylcoumarin (Z­GP­AMC) to Z­Gly­Pro and 7­amino­4­methylcoumarin (AMC) and the specific activity is >600 pmol/min/µg.

Cynomolgus

HEK293

N-His

XP_005573377.1 (R30-D760)

102134935  [NCBI]

RVHNSEENTMRALTLKDILNGTFSYKTFFPNWISGQEYLHQSADNNIVLYNIETGQSYTILSNRTMKSVNASNYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLSNGEFVRGNELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITFNGRENKIFNGIPDWVYEEEMLATKYALWWSPNGKFLAYAEFNDTDIPVIAYSYYGDEQYPRTINIPYPKAGAKNPFVRIFIIDTTYPAYVGPQEVPVPAMIASSDYYFSWLTWVTDERVCLQWLKRVQNVSVLSICDFREDWQTWDCPKTQEHIEESRTGWAGGFFVSTPVFSYDAISYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAINIFRVTQDSLFYSSNEFEDYPGRRNIYRISIGSYPPSKKCVTCHLRKERCQYYTASFSDYAKYYALVCYGPGIPISTLHDGRTDQEIKILEENKELENALKNIQLPKEEIKKLEVDEITLWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVRSVFAVNWISYLASKEGMVIALVDGRGTAFQGDKLLYAVYRKLGVYEVEDQITAVRKFIEMGFIDEKRIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASVYTERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGLSGLSTNHLYTHMTHFLKQCFSLSD

Approximately 87 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.