FDFT1 蛋白, Human (His)

FDFT1 is a key enzyme in cellular metabolism responsible for coordinating the condensation of two farnesyl pyrophosphate (FPP) molecules, a key step in sterol biosynthesis. The process proceeds in two distinct steps: First, two FPP molecules react to form the stable squalene diphosphate intermediate (PSQPP), releasing protons and inorganic diphosphate. FDFT1 Protein, Human (His) is the recombinant human-derived FDFT1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of FDFT1 Protein, Human (His) is 416 a.a., with molecular weight of ~52 kDa.

FDFT1（或角鲨烯合酶）是一种重要的酶，在甾醇生物合成中发挥着关键作用。FDFT1 作为该途径中的第一个关键酶，通过两步过程催化两个法尼基焦磷酸 (FPP) 分子缩合形成角鲨烯。在最初的半反应中，两个 FPP 分子反应生成稳定的角鲨烯二磷酸中间体 (PSQPP)，同时释放出质子和无机二磷酸盐。随后，在第二个半反应中，PSQPP 发生杂解、异构化，并与 NADPH 或 NADH 还原，最终产生角鲨烯。这种酶促过程是甾醇合成的基础，突显了 FDFT1 在控制甾醇生物合成初始步骤中的核心作用。

Human

E. coli

N-6*His

P37268 (E2-H417)

2222  [NCBI]

EFVKCLGHPEEFYNLVRFRIGGKRKVMPKMDQDSLSSSLKTCYKYLNQTSRSFAAVIQALDGEMRNAVCIFYLVLRALDTLEDDMTISVEKKVPLLHNFHSFLYQPDWRFMESKEKDRQVLEDFPTISLEFRNLAEKYQTVIADICRRMGIGMAEFLDKHVTSEQEWDKYCHYVAGLVGIGLSRLFSASEFEDPLVGEDTERANSMGLFLQKTNIIRDYLEDQQGGREFWPQEVWSRYVKKLGDFAKPENIDLAVQCLNELITNALHHIPDVITYLSRLRNQSVFNFCAIPQVMAIATLAACYNNQQVFKGAVKIRKGQAVTLMMDATNMPAVKAIIYQYMEEIYHRIPDSDPSSSKTRQIISTIRTQNLPNCQLISRSHYSPIYLSFVMLLAALSWQYLTTLSQVTEDYVQTGEH

Approximately 52 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.