FGFR-1 beta 蛋白, Human (HEK293, hFc)

FGFR-1 beta is a member of the FGFR family that interacts with fibroblast growth factors and regulates mitosis and differentiation. It specifically binds acidic and basic fibroblast growth factors and is involved in limb induction. FGFR-1 beta Protein, Human (HEK293, hFc) is the recombinant human-derived FGFR-1 beta protein, expressed by HEK293 , with C-hFc labeled tag. The total length of FGFR-1 beta Protein, Human (HEK293, hFc) is 264 a.a., with molecular weight of 97.5-110 kDa.

FGFR-1 β 蛋白是成纤维细胞生长因子受体 (FGFR) 家族中高度保守的成员，与其他成员具有相似的氨基酸序列。FGFR 家族成员对配体表现出不同的亲和力，并分布在各种组织中。全长蛋白质由具有三个免疫球蛋白样结构域的胞外区、疏水性跨膜片段和胞质酪氨酸激酶结构域组成。细胞外部分与成纤维细胞生长因子相互作用，启动调节有丝分裂和分化的下游信号。FGFR-1 β 特异性结合酸性和碱性成纤维细胞生长因子，并在肢体诱导中发挥作用。该基因的突变与多种综合征和疾病有关，包括 Pfeiffer 综合征、Jackson-Weiss 综合征、Antley-Bixler 综合征、骨发育不良和常染色体显性卡尔曼综合征 2。涉及该基因的染色体畸变与干细胞骨髓增殖性疾病和干细胞白血病淋巴瘤综合征。编码不同蛋白质亚型的各种可变剪接变体已被鉴定，尽管并非所有变体都已被完全表征。FGFR-1 β 蛋白在多种组织中普遍表达，包括卵巢、脂肪和 25 种其他组织。

Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 1.096 ng/mL in the presence of 1ng/mL human FGF-1, corresponding to a specific activity is 9.12×10^5 units/mg.

Human

HEK293

C-hFc

NP_075594.1 (R22-E285)

2260  [NCBI]

RPSPTLPEQDALPSSEDDDDDDDSSSEEKETDNTKPNPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLE

97.5-110 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.