FKTN 蛋白, Human (Cell-Free, His)

The FKTN protein catalyzes the transfer of ribitol phosphate from CDP-ribitol to the distal N-acetylgalactosamine in the phosphorylated O-mannosyl trisaccharide of α-dystroglycan (DAG1). FKTN Protein, Human (Cell-Free, His) is the recombinant human-derived FKTN protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of FKTN Protein, Human (Cell-Free, His) is 461 a.a., with molecular weight of 56.5 kDa.

FKTN 蛋白在催化磷酸核糖醇从 CDP-核糖醇转移到 α-肌营养不良聚糖 (DAG1) 中发现的磷酸化 O-甘露糖基三糖的远端 N-乙酰半乳糖胺方面发挥着至关重要的作用。这种酶活性代表了形成核糖醇 5-磷酸串联重复序列的初始步骤，将磷酸化的 O-甘露糖基三糖连接到由 3-木糖基-α-1,3-葡萄糖醛酸-β-重复序列组成的配体结合部分。1. FKTN 对于维持 POMGNT1 在高尔基膜中的正常定位并确保其正常活性至关重要。此外，FKTN 可能参与与包含外部和内部肌肉膜的大型复合体相互作用并增强该复合体。与大脑发育也有潜在的联系。

Human

E. coli Cell-free

N-10*His

O75072 (M1-Y461)

2218

MSRINKNVVLALLTLTSSAFLLFQLYYYKHYLSTKNGAGLSKSKGSRIGFDSTQWRAVKKFIMLTSNQNVPVFLIDPLILELINKNFEQVKNTSHGSTSQCKFFCVPRDFTAFALQYHLWKNEEGWFRIAENMGFQCLKIESKDPRLDGIDSLSGTEIPLHYICKLATHAIHLVVFHERSGNYLWHGHLRLKEHIDRKFVPFRKLQFGRYPGAFDRPELQQVTVDGLEVLIPKDPMHFVEEVPHSRFIECRYKEARAFFQQYLDDNTVEAVAFRKSAKELLQLAAKTLNKLGVPFWLSSGTCLGWYRQCNIIPYSKDVDLGIFIQDYKSDIILAFQDAGLPLKHKFGKVEDSLELSFQGKDDVKLDVFFFYEETDHMWNGGTQAKTGKKFKYLFPKFTLCWTEFVDMKVHVPCETLEYIEANYGKTWKIPVKTWDWKRSPPNVQPNGIWPISEWDEVIQLY

56.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.