1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. FRK 蛋白, Human (Sf9, GST)

FRK 蛋白, Human (Sf9, GST)

目录号: HY-P701688
产品使用指南

FRK 蛋白是一种非受体酪氨酸蛋白激酶,通过正向调节 PTEN 蛋白稳定性来充当细胞增殖的负调节因子。通过“Tyr-336”上的 PTEN 磷酸化,FRK 可以通过减少其与 NEDD4 的结合来防止 PTEN 泛素化和降解。FRK 蛋白, Human (Sf9, GST) 是重组的 FRK 蛋白,由 Sf9 insect cells 表达,带有 N-GST 标签。FRK 蛋白, Human (Sf9, GST) 全长 504 个氨基酸。

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描述

FRK protein is a non-receptor tyrosine protein kinase that acts as a negative regulator of cell proliferation by positively regulating PTEN protein stability. Through phosphorylation of PTEN on “Tyr-336”, FRK may prevent PTEN ubiquitination and degradation by reducing its binding to NEDD4. FRK Protein, Human (Sf9, GST) is the recombinant human-derived FRK protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of FRK Protein, Human (Sf9, GST) is 504 a.a., .

研究背景

FRK 蛋白是一种非受体酪氨酸蛋白激酶,发挥细胞增殖负调节因子的作用。其关键功能涉及通过“Tyr-336”上 PTEN 的磷酸化实现 PTEN 蛋白稳定性的正向调节。这种磷酸化事件可能通过减少 PTEN 与 NEDD4 的结合来防止 PTEN 泛素化和降解。FRK 蛋白增强 PTEN 稳定性的能力表明了一种抵消过度细胞增殖的调节机制,这意味着 FRK 蛋白在细胞过程中具有潜在的肿瘤抑制作用。

种属

Human

表达系统

Sf9 insect cells

标签

N-GST

蛋白编号

P42685 (S2-R505)

基因 ID

2444

蛋白结构
N-term
GST
FRK (S2-R505)
Accession # P42685
C-term
同用名
FRK; Tyrosine-protein kinase FRK; FYN-related kinase; Nuclear tyrosine protein kinase RAK; Protein-tyrosine kinase 5
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

FRK Protein, Human (Sf9, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
FRK Protein, Human (Sf9, GST)
目录号:
HY-P701688
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