GABARAPL2/GATE-16 蛋白, Human (His)

GABARAPL2/GATE-16 is an important ubiquitin-like modifier that complexly regulates intra-Golgi flux by coupling NSF activity with SNARE activation. It stimulates the ATPase activity of NSF and promotes binding to GOSR1. GABARAPL2/GATE-16 Protein, Human (His) is the recombinant human-derived GABARAPL2/GATE-16 protein, expressed by E. coli , with N-6*His labeled tag. The total length of GABARAPL2/GATE-16 Protein, Human (His) is 117 a.a., with molecular weight of ~15 KDa.

GABARAPL2/GATE-16 是一种泛素样修饰剂，复杂地参与高尔基体内的运输，通过 NSF 活性和 SNARE 激活的耦合来调节运输。它刺激 NSF 的 ATP 酶活性，促进其与 GOSR1 的结合。除了与高尔基体相关的功能外，GABARAPL2/GATE-16 在自噬中发挥着至关重要的作用，通过参与线粒体自噬有助于线粒体质量控制。与参与吞噬细胞膜伸长的 LC3 不同，GABARAP/GATE-16 亚家族在自噬体成熟的后期阶段发挥着关键作用。作为单体，GABARAPL2/GATE-16 与关键自噬相关蛋白（如 ATG3、ATG7、ATG13、ULK1、TP53INP1、TP53INP2、TBC1D25、SQSTM1、BNIP3、TECPR2、PCM1、TBC1D5、TRIM5、MEFV）建立相互作用网络、TRIM21、WDFY3、UBA5、GOSR1、KBTBD6、KBTBD7 等。这些相互作用强调了它在细胞过程中的多功能作用，从高尔基动力学到自噬和网状自噬调节。

Data is not available

Human

E. coli

N-6*His

P60520 (M1-F117)

11345  [NCBI]

MKWMFKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKRKYLVPSDITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEKDEDGFLYVAYSGENTFGF

Approximately 15 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from sterile 50 mM Tris, 300 mM NaCl, pH 7.4, 5% trehalose,5% mannitol and 0.01% Tween80 .

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.