GAPDH 蛋白, Human (His-SUMO)

GAPDH, a multifunctional protein, acts as a key enzyme in glycolysis, catalyzing the conversion of G3P. It also functions as a nitrosylase, impacting nuclear processes. In addition, it influences cytoskeleton organization, associates with CHP1, participates in transcript-selective translation inhibition, contributes to immune responses, and mediates nuclear events through S-nitrosylation of target proteins. GAPDH Protein, Human (His-SUMO) is the recombinant human-derived GAPDH protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of GAPDH Protein, Human (His-SUMO) is 334 a.a., with molecular weight of ~51.9 kDa.

3-磷酸甘油醛脱氢酶 (GAPDH) 具有双重功能，既作为糖酵解中的关键酶，催化 D-甘油醛 3-磷酸 (G3P) 转化为 3-磷酸-D-甘油酰磷酸，又作为亚硝基酶，具有在核功能中的作用。在糖酵解过程中，GAPDH 在该途径的初始步骤中发挥着关键作用。除了其代谢功能外，GAPDH 还调节细胞骨架的组织和组装，通过与 CHP1 相互作用促进微管和膜的关联。此外，它是步态复合体的一个组成部分，参与炎症过程中干扰素-γ诱导的转录选择性翻译抑制。在先天免疫领域，GAPDH 分别通过与 TRAF2 和 TRAF3 相互作用，促进 TNF 诱导的 NF-κ-B 激活和 I 型干扰素产生。此外，GAPDH 还参与各种核事件，包括转录、RNA 转运、DNA 复制和细胞凋亡，其亚硝基酶活性介导核靶蛋白（如 SIRT1、HDAC2 和 PRKDC）的半胱氨酸 S-亚硝基化。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His;N-SUMO

P04406 (G2-E335)

2597  [NCBI]

GKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE

Approximately 51.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.