GFPT1 蛋白, Human

As a key regulator of glucose influx into the hexosamine pathway, the GFPT1 protein plays a key role in controlling the availability of protein N- and O-linked glycosylation precursors. In addition, GFPT1 is involved in the regulation of circadian expression of clock genes BMAL1 and CRY1 and contributes to the complex regulation of cytoplasmic UDP-GlcNAc metabolic fluctuations. GFPT1 Protein, Human is the recombinant human-derived GFPT1 protein, expressed by E. coli , with tag free. The total length of GFPT1 Protein, Human is 368 a.a., with molecular weight of ~41.5 KDa.

GFPT1 蛋白对葡萄糖进入己糖胺途径的流量进行控制，并且可能对于调节蛋白质 N- 和 O- 连接糖基化所需的前体的可用性至关重要。此外，它在 BMAL1 和 CRY1 等时钟基因的昼夜节律调节中发挥作用，表明它参与调节昼夜节律。此外，GFPT1 还参与微调胞质 UDP-GlcNAc 的代谢波动，特别是影响其在组织重塑过程中对透明质酸合成的影响。这些不同的功能强调了 GFPT1 在协调与糖基化、昼夜节律和组织重塑相关的代谢过程和细胞反应中的重要性。

Measured by its ability to catalyzes the reaction of glutamine and F-6-P to produce glutamate and GlcN-6-P. The specific activity is 1.038 nmoL/min/mg.

Human

E. coli

Tag Free

Q06210 (Q332-E699)

2673  [NCBI]

QQIMKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYTVNLGGLKDHIKEIQRCRRLILIACGTSYHAGVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFLSQSGETADTLMGLRYCKERGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMFALMMCDDRISMQERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMGRGYHYATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE

Approximately 41.5 kDa.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.