GFRA1/GDNFR-alpha-1 蛋白, Rat (HEK293, Fc)

GFRA1/GDNFR-α-1 protein is a receptor for GDNF and critically mediates GDNF-induced autophosphorylation and RET receptor activation. Presumably, two GDNFR-α-1 molecules form a complex with disulfide-linked GDNF, two RET molecules, and SORL1, suggesting the existence of a coordinated signaling mechanism. GFRA1/GDNFR-alpha-1 Protein, Rat (HEK293, Fc) is the recombinant rat-derived GFRA1/GDNFR-alpha-1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of GFRA1/GDNFR-alpha-1 Protein, Rat (HEK293, Fc) is 445 a.a., with molecular weight of ~85 kDa.

GFRA1/GDNFR-α-1 蛋白作为 GDNF 的受体，在介导 GDNF 诱导的自身磷酸化和 RET 受体的激活中发挥着至关重要的作用。有人提出，两个分子的 GDNFR-α-1 与二硫键连接的 GDNF 二聚体和两个分子的 RET 形成复合物，这表明信号转导的协调机制。GFRA1 与 RET 相互作用，促进与 GDNF 诱导的反应相关的复杂信号通路。此外，GFRA1 与 SORL1 相互作用，无论是单独的还是与 GDNF 形成复合物。这种相互作用导致 GFRA1 内化，而不导致其降解，表明 GFRA1 动态的调节方面。这些分子相互作用凸显了 GFRA1 在介导 GDNF 信号传导中的多方面作用，并强调了其在细胞过程中的重要性。

Immobilized rat His-GDNF (78-211) at 10 μg/mL (100 μl/well) can bind rat GFRA1-Fc, The EC₅₀ of rat GFRA1-Fc is 20-46.6 ng/mL.

Rat

HEK293

C-hFc

Q62997 (M1-L445)

25454  [NCBI]

MFLATLYFALPLLDLLMSAEVSGGDRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLTSGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRAVPFISDVFQQVEHISKGNNCLDAAKACNLDDTCKKYRSAYITPCTTSMSNEVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEERERPNCLSLQDSCKTNYICRSRLADFFTNCQPESRSVSNCLKENYADCLLAYSGLIGTVMTPNYVDSSSLSVAPWCDCSNSGNDLEDCLKFLNFFKDNTCLKNAIQAFGNGSDVTMWQPAPPVQTTTATTTTAFRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGSTHLCLSDSDFGKDGLAGASSHITTKSMAAPPSCSL

Approximately 85 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.