2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. GOT1 蛋白, Human (His)

GOT1 蛋白, Human (His)

目录号: HY-P7611
COA 产品使用指南

GOT1 蛋白, Human (His)是一种在 E. coli 体系中表达的，末端有 His 标签的重组人 GOT1。血液中 GOT1 的活性可诱导血液中谷氨酸浓度的降低，GOT1 催化草酰乙酸和谷氨酸可逆转化为天冬氨酸和 α-酮戊二酸。

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规格 价格 是否有货
10 μg ￥1150 询问价格 & 货期
50 μg ￥3450 询问价格 & 货期

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描述

GOT1 Protein, Human (His), a recombinant human GOT1 produced in E. coli, has a His tag. Calcitonin has an effect on decreasing osteoclast activity and has the potential for hypercalcemia research. Reduction in glutamate concentration in blood is induced through the activity of the blood-resident enzyme glutamate oxaloacetate transaminase 1 (GOT1), which catalyzes the reversible transformation of oxaloacetate and glutamate to aspartate and α-ketoglutarate[1].
研究背景

血液谷氨酸清除是一种新颖且有吸引力的保护策略，可减少缺血性脑损伤期间释放的细胞外谷氨酸的兴奋毒性作用。通过施用草酰乙酸激活谷氨酸草酰乙酸转氨酶 1 (GOT1)，已被用于降低血液中谷氨酸浓度[1]。
种属

Human
表达系统

E. coli
标签

C-6*His
蛋白编号

P17174 (A2-Q413)
基因 ID
蛋白结构
N-term
GOT1 (A2-Q413)
Accession # P17174
6*His
C-term
同用名
rHuGOT1, His; Glutamate Oxaloacetate Transaminase 1; Transaminase A; GOT1
氨基酸序列

APPSVFAEVPQAQPVLVFKLTADFREDPDPRKVNLGVGAYRTDDCHPWVLPVVKKVEQKIANDNSLNHEYLPILGLAEFRSCASRLALGDDSPALKEKRVGGVQSLGGTGALRIGADFLARWYNGTNNKNTPVYVSSPTWENHNAVFSAAGFKDIRSYRYWDAEKRGLDLQGFLNDLENAPEFSIVVLHACAHNPTGIDPTPEQWKQIASVMKHRFLFPFFDSAYQGFASGNLERDAWAIRYFVSEGFEFFCAQSFSKNFGLYNERVGNLTVVGKEPESILQVLSQMEKIVRITWSNPPAQGARIVASTLSNPELFEEWTGNVKTMADRILTMRSELRARLEALKTPGTWNHITDQIGMFSFTGLNPKQVEYLVNEKHIYLLPSGRINVSGLTTKNLDYVATSIHEAVTKIQHHHHHH

分子量

Approximately 45.0 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料

COA (208 KB)

产品使用指南 (1213 KB)
参考文献

GOT1 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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