GSK-3 beta 蛋白, Human (P. pastoris, His)

GSK-3 beta is an active protein kinase that regulates a variety of cellular processes. It negatively regulates glucose homeostasis, Wnt signaling, and transcription factors by phosphorylating substrates such as glycogen synthase, CTNNB1, and JUN. GSK-3 beta Protein, Human (P. pastoris, His) is the recombinant human-derived GSK-3 beta protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of GSK-3 beta Protein, Human (P. pastoris, His) is 420 a.a., with molecular weight of 48.7 kDa.

GSK-3 beta 是一种组成型活性蛋白激酶，在调节多种细胞过程中发挥多方面的作用。它作为葡萄糖稳态、Wnt 信号传导和转录因子的激素控制的负调节因子，通过磷酸化和失活关键底物（如糖原合酶、EIF2B、CTNNB1/β-连环蛋白、APC、AXIN1、DPYSL2/CRMP2、 JUN、NFATC1/NFATC、MAPT/TAU 和 MACF1。在骨骼肌中，它有助于胰岛素对糖原合成的调节。GSK-3 beta 参与 Wnt 信号传导导致 CTNNB1 降解，而其 JUN 和 NFATC1/NFATC 的磷酸化则调节它们的活性。该激酶还在微管动力学中发挥着至关重要的作用，影响 MAPT/TAU 稳定性并有助于 ERBB2 依赖性微管稳定。此外，GSK-3 beta 通过与 MCL1 和 SNAI1 相互作用参与多种途径，例如 NF-kappa-B 调节、胰腺 β 细胞复制和凋亡控制。此外，它还协调昼夜节律、自噬和外源性细胞凋亡信号传导，突出了其在细胞稳态和对外部刺激的反应中的核心作用。

Human

P. pastoris

N-6*His

P49841 (M1-T420)

2932  [NCBI]

MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASASNST

48.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.