GULO 蛋白, Rat (Cell-Free, His)

GULO Protein facilitates the oxidation of L-gulono-1,4-lactone, generating hydrogen peroxide and L-xylo-hexulonolactone. The latter undergoes spontaneous isomerization, leading to the production of L-ascorbate, commonly known as vitamin C. GULO's enzymatic role in this pathway underscores its significance in the cellular synthesis of L-ascorbate, a crucial antioxidant supporting redox balance and essential physiological functions. GULO Protein, Rat (Cell-Free, His) is the recombinant rat-derived GULO protein, expressed by E. coli Cell-free , with C-10*His labeled tag. The total length of GULO Protein, Rat (Cell-Free, His) is 439 a.a., with molecular weight of 52.0 kDa.

GULO 蛋白催化 L-古洛糖酸-1,4-内酯的氧化，从而产生过氧化氢和 L-木己糖酸内酯。后一种化合物自发地发生异构化，最终产生L-抗坏血酸。这一酶促过程凸显了 GULO 在 L-抗坏血酸（通常称为维生素 C，多种生物系统中的重要抗氧化剂）生物合成中的关键作用。GULO 促进这种氧化途径的能力有助于 L-抗坏血酸的细胞合成，强调其在维持氧化还原平衡和支持基本生理功能方面的重要性。

Rat

E. coli Cell-free

C-10*His

P10867 (V2-Y440)

60671

VHGYKGVQFQNWAKTYGCSPEVYYQPTSVEEVREVLALAREQKKKVKVVGGGHSPSDIACTDGFMIHMGKMNRVLQVDKEKKQITVEAGILLADLHPQLDEHGLAMSNLGAVSDVTVAGVIGSGTHNTGIKHGILATQVVALTLMTADGEVLECSESRNADVFQAARVHLGCLGIILTVTLQCVPQFHLQETSFPSTLKEVLDNLDSHLKRSEYFRFLWFPHTENVSIIYQDHTNKAPSSASNWFWDYAIGFYLLEFLLWTSTYLPCLVGWINRFFFWMLFNCKKESSNLSHKIFTYECRFKQHVQDWAIPREKTKEALLELKAMLEAHPKVVAHYPVEVRFTRGDDILLSPCFQRDSCYMNIIMYRPYGKDVPRLDYWLAYETIMKKFGGRPHWAKAHNCTQKDFEEMYPTFHKFCDIREKLDPTGMFLNSYLEKVFY

52.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.