GZMA/Granzyme A 蛋白, Human (HEK293, His)

GZMA/granzyme A is enriched in cytotoxic T cells and natural killer cells and can induce caspase-independent pyroptosis after delivery to target cells. With substrate specificity for lysine or arginine residues, GZMA cleaves APEX1 and the nucleosome assembly protein SET, thereby disrupting their activity. GZMA/Granzyme A Protein, Human (HEK293, His) is the recombinant human-derived GZMA/Granzyme A protein, expressed by HEK293 , with C-His labeled tag. The total length of GZMA/Granzyme A Protein, Human (HEK293, His) is 236 a.a., with molecular weight of ~27-33 KDa.

粒酶 A (GZMA) 是一种在细胞毒性 T 细胞和自然杀伤细胞的胞质颗粒中发现的高度丰富的蛋白酶，在免疫防御机制中发挥着至关重要的作用。当通过免疫突触递送至靶细胞时，GZMA 会激活不依赖于 caspase 的细胞焦亡。它对赖氨酸或精氨酸残基后的裂解表现出底物特异性。值得注意的是，GZMA 在“Lys-31”之后裂解 APEX1，破坏其氧化修复活性。此外，它还针对核小体组装蛋白 SET，在“Lys-189”之后将其切割。这种裂解事件破坏了 SET 的核小体组装活性，并促进 SET 复合物易位到细胞核中，在细胞核中参与切口和降解 DNA。GZMA 的多功能活性强调了其在免疫反应过程中协调多种细胞过程的重要性。

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5'-Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >10875 pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-His

NP_006135.1 (E27-V262)

3001  [NCBI]

EKIIGGNEVTPHSRPYMVLLSLDRKTICAGALIAKDWVLTAAHCNLNKRSQVILGAHSITREEPTKQIMLVKKEFPYPCYDPATREGDLKLLQLTEKAKINKYVTILHLPKKGDDVKPGTMCQVAGWGRTHNSASWSDTLREVNITIIDRKVCNDRNHYNFNPVIGMNMVCAGSLRGGRDSCNGDSGSPLLCEGVFRGVTSFGLENKCGDPRGPGVYILLSKKHLNWIIMTIKGAV

Approximately 27-33 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.