HDAC8 蛋白, Human (sf9, GST)

The HDAC8 protein is a histone deacetylase that has been shown to be critical for lysine deacetylation on core histones (H2A, H2B, H3, H4). This enzymatic activity initiates epigenetic repression that affects transcription, cell cycle, and developmental events. HDAC8 Protein, Human (sf9, GST) is the recombinant human-derived HDAC8 protein, expressed by Sf9 insect cells , with C-GST labeled tag. The total length of HDAC8 Protein, Human (sf9, GST) is 377 a.a., with molecular weight of ~68 kDa.

HDAC8 蛋白是一种组蛋白脱乙酰酶，可催化位于核心组蛋白 N 末端区域（即 H2A、H2B、H3 和 H4）的赖氨酸残基的脱乙酰化，这一点已得到多项研究的支持。这种酶活性对于启动表观遗传抑制至关重要，在转录调节、细胞周期进程和发育事件中发挥着关键作用。HDAC8 等组蛋白脱乙酰酶在大型多蛋白复合物中发挥作用，有助于染色质结构和功能的复杂调节。此外，HDAC8 参与粘连蛋白复合物蛋白 SMC3 的脱乙酰化，影响粘连蛋白复合物从染色质的释放。值得注意的是，HDAC8 也可能在平滑肌细胞收缩性中发挥作用。HDAC8 扩展了其酶库，表现出蛋白质赖氨酸脱酰酶活性，通过介导组蛋白的去巴豆酰化，特别是赖氨酸残基（(2E)-丁烯酰基）的巴豆酰化，特别发挥蛋白质去巴豆酰化酶的作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

C-GST

Q9BY41 (M1-V377)

55869  [NCBI]

Approximately 68 kDa

Greater than 88% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.