1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. HDAC8 蛋白, Mouse (sf9, His)

HDAC8 蛋白, Mouse (sf9, His)

目录号: HY-P75144
产品使用指南

HDAC8 蛋白是一种组蛋白脱乙酰酶,可去除核心组蛋白赖氨酸残基上的乙酰基,有助于表观遗传抑制并影响转录调控、细胞周期进程和发育事件。它形成大型多蛋白复合物并使 SMC3 脱乙酰化,调节粘连蛋白复合物的释放。HDAC8 蛋白, Mouse (sf9, His) 是重组的 HDAC8 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。HDAC8 蛋白, Mouse (sf9, His) 全长 377 个氨基酸,分子量约为 ~46 kDa。

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描述

The HDAC8 protein is a histone deacetylase that removes acetyl groups from core histone lysine residues, contributing to epigenetic repression and affecting transcriptional regulation, cell cycle progression, and developmental events. It forms large multiprotein complexes and deacetylates SMC3, regulating the release of cohesin complexes. HDAC8 Protein, Mouse (sf9, His) is the recombinant mouse-derived HDAC8 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of HDAC8 Protein, Mouse (sf9, His) is 377 a.a., with molecular weight of ~46 kDa.

研究背景

HDAC8 蛋白是一种组蛋白脱乙酰酶,有助于去除核心组蛋白(H2A、H2B、H3 和 H4)N 端部分赖氨酸残基上的乙酰基。这种脱乙酰化过程有助于表观遗传抑制,从而对转录调控、细胞周期进程和发育事件产生重大影响。HDAC8 的活性是通过形成大型多蛋白复合物来介导的。此外,它还参与粘连蛋白复合物蛋白 SMC3 的脱乙酰化,从而调节粘连蛋白复合物从染色质的释放。HDAC8 也可能在平滑肌细胞收缩性中发挥作用。此外,除了其脱乙酰酶功能外,HDAC8 通过充当蛋白质去巴豆酰化酶,催化去除组蛋白中的巴豆酰基((2E)-丁烯酰基),从而表现出蛋白质赖氨酸脱酰酶活性。

种属

Mouse

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

Q8VH37 (M1-V377)

基因 ID
蛋白结构
N-term
HDAC8 (M1-V377)
Accession # Q8VH37
His
C-term
同用名
Histone deacetylase 8; HD8; HDAC8; HDACL1; CDA07
分子量

Approximately 46 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HDAC8 Protein, Mouse (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HDAC8 Protein, Mouse (sf9, His)
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