1. 重组蛋白
  2. Others
  3. HFE 蛋白, Mouse (Cell-Free, His)

HFE 蛋白, Mouse (Cell-Free, His)

目录号: HY-P702321
产品使用指南

HFE 蛋白至关重要,它与转铁蛋白受体 (TFR) 结合并降低其与载铁转铁蛋白的亲和力。这种相互作用依赖于 pH 值并由 HFE 的胞外结构域介导,凸显了复杂的分子机制。通过调节 TFR 的亲和力,HFE 在调节铁稳态方面发挥着关键作用,强调了其在与铁吸收和代谢相关的细胞过程中的重要性。HFE 蛋白, Mouse (Cell-Free, His) 是重组的 HFE 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。HFE 蛋白, Mouse (Cell-Free, His) 全长 335 个氨基酸,分子量为 39.5 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

HFE Protein is crucial, binding to transferrin receptor (TFR) and reducing its affinity for iron-loaded transferrin. The interaction, pH-dependent and mediated by HFE's extracellular domain, highlights intricate molecular mechanisms. By modulating TFR's affinity, HFE plays a pivotal role in regulating iron homeostasis, emphasizing its significance in cellular processes related to iron uptake and metabolism. HFE Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived HFE protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of HFE Protein, Mouse (Cell-Free, His) is 335 a.a., with molecular weight of 39.5 kDa.

研究背景

HFE 蛋白发挥着至关重要的作用,因为它与转铁蛋白受体 (TFR) 结合,有效降低其与载铁转铁蛋白的亲和力。这种相互作用通过 HFE 的胞外结构域以 pH 依赖性方式发生,强调了所涉及分子机制的复杂性。通过调节 TFR 与铁负载转铁蛋白的亲和力,HFE 在铁稳态调节中发挥着关键作用,显示出其在与铁吸收和代谢相关的细胞过程中的重要性。

种属

Mouse

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

P70387 (Q25-E359)

基因 ID

15216

蛋白结构
N-term
10*His
HFE (Q25-E359)
Accession # P70387
C-term
同用名
Hereditary hemochromatosis protein homolog
氨基酸序列

QALPPRSHSLRYLFMGASEPDLGLPLFEARGYVDDQLFVSYNHESRRAEPRAPWILEQTSSQLWLHLSQSLKGWDYMFIVDFWTIMGNYNHSKVTKLGVVSESHILQVVLGCEVHEDNSTSGFWRYGYDGQDHLEFCPKTLNWSAAEPGAWATKVEWDEHKIRAKQNRDYLEKDCPEQLKRLLELGRGVLGQQVPTLVKVTRHWASTGTSLRCQALDFFPQNITMRWLKDNQPLDAKDVNPEKVLPNGDETYQGWLTLAVAPGDETRFTCQVEHPGLDQPLTASWEPLQSQAMIIGIISGVTVCAIFLVGILFLILRKRKASGGTMGGYVLTDCE

分子量

39.5 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HFE Protein, Mouse (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
HFE Protein, Mouse (Cell-Free, His)
目录号:
HY-P702321
需求量: