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Histone H3 蛋白, Xenopus laevis (98a.a)

目录号: HY-P72334
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组蛋白 H3 蛋白构成核小体的核心元件,是由 H2A、H2B、H3 和 H4 组成的八聚体。该组装体与一个 H3-H4 异四聚体和两个 H2A-H2B 异二聚体形成组蛋白八聚体,作为包裹 147 个 DNA 碱基对的分子线轴。这种紧凑的组织有助于染色质结构,而组蛋白 H3 作为该组装的一部分,在染色质结构和基因调控中发挥着至关重要的作用。Histone H3 蛋白, Xenopus laevis (98a.a) 是重组的 组蛋白 H3 蛋白,由 E. coli 表达,不带标签。Histone H3 蛋白, Xenopus laevis (98a.a) 全长 98 个氨基酸,分子量约为 ~11.5 kDa。

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描述

Histone H3 Protein constitutes a core element in the nucleosome, an octamer comprising H2A, H2B, H3, and H4. The assembly forms a histone octamer with one H3-H4 heterotetramer and two H2A-H2B heterodimers, serving as a molecular spool that wraps around 147 base pairs of DNA. This compact organization contributes to chromatin structure, and Histone H3, as part of this assembly, plays a crucial role in chromatin structure and gene regulation. Histone H3 Protein, Xenopus laevis (98a.a) is the recombinant Xenopus laevis-derived Histone H3 protein, expressed by E. coli , with tag free. The total length of Histone H3 Protein, Xenopus laevis (98a.a) is 98 a.a., with molecular weight of ~11.5 kDa.

研究背景

组蛋白 H3 蛋白是核小体的基本成分,由组蛋白八聚体组成,其中每个分子包含 H2A、H2B、H3 和 H4 两个分子。该组装体由一个 H3-H4 异四聚体和两个 H2A-H2B 异二聚体组成,共同形成八聚体核心。组蛋白八聚体起到分子线轴的作用,将大约 147 个 DNA 碱基对包裹在自身周围,有助于染色质的紧凑组织。组蛋白 H3 作为该组装体的一部分,属于组蛋白 H3 家族,在染色质结构和基因调控中发挥着关键作用。

种属

Xenopus laevis

表达系统

E. coli

标签

Tag Free

蛋白编号

Q92133 (P39-A136)

基因 ID

399088  [NCBI]

蛋白结构
N-term
Histone H3 (P39-A136)
Accession # Q92133
C-term
同用名
H3c8.S; H3I; hist1h3g
氨基酸序列

PHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVALFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

分子量

Approximately 11.5 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Histone H3 Protein, Xenopus laevis (98a.a) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Histone H3 Protein, Xenopus laevis (98a.a)
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HY-P72334
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