HK1/Hexokinase-1 蛋白, Human (463a.a, His)

The HK1/hexokinase-1 protein phosphorylates hexose sugars, including D-glucose, D-glucosamine, D-fructose, D-mannose, and 2-deoxy-D-glucose, to form the corresponding hexose 6-phosphate. HK1/Hexokinase-1 Protein, Human (463a.a, His) is the recombinant human-derived HK1/Hexokinase-1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of HK1/Hexokinase-1 Protein, Human (463a.a, His) is 463 a.a., with molecular weight of 55.9 kDa.

己糖激酶-1 (HK1) 蛋白通过催化多种己糖（包括 D-葡萄糖、D-葡萄糖胺、D-果糖、D-甘露糖和 2-脱氧-D-葡萄糖）的磷酸化，在细胞代谢中发挥关键作用。生成6-磷酸己糖（分别为D-葡萄糖6-磷酸、D-葡萄糖胺6-磷酸、D-果糖6-磷酸、D-甘露糖6-磷酸和2-脱氧-D-葡萄糖6-磷酸）。虽然 HK1 不会磷酸化 N-乙酰基-D-葡萄糖胺，但它通过催化 D-葡萄糖磷酸化为 D-葡萄糖 6-磷酸来介导糖酵解的关键初始步骤。除了其代谢功能外，HK1 还参与先天免疫和炎症，充当细菌肽聚糖的模式识别受体。在细胞质中，细菌肽聚糖的 N-乙酰基-D-葡萄糖胺成分抑制 HK1 的活性，导致其从线粒体外膜解离，随后激活 NLRP3 炎性小体。这种多方面的作用强调了 HK1 在糖酵解之外的细胞过程中的重要性，强调了它在免疫反应和炎症中的参与。

Measured by its ability to phosphorylate glucose. The specific activity is 385.882 pmol/min/μg, as measured under the described conditions.

Human

E. coli

N-6*His

P19367-1 (E13-A475)

3098  [NCBI]

ELKDDQVKKIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLA

55.9 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of Tris/PBS-based buffer, 6% Trehalose, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.