HSP27/HSPB1 蛋白, Human (His)

The HSP27/HSPB1 protein is a small heat shock protein that acts as a molecular chaperone, possibly maintaining denatured proteins in a foldable state. In addition to its chaperone role, it crucially enhances stress resistance and contributes to actin organization. HSP27/HSPB1 Protein, Human (His) is the recombinant human-derived HSP27/HSPB1 protein, expressed by E. coli , with C-His labeled tag. The total length of HSP27/HSPB1 Protein, Human (His) is 205 a.a., with molecular weight of ~28 kDa.

HSP27/HSPB1 是一种小型热休克蛋白，作为分子伴侣，可能在维持变性蛋白质处于可折叠状态中发挥作用。除了其伴侣功能外，这种蛋白质在抗应激方面也发挥着至关重要的作用，并有助于肌动蛋白的组织。其分子伴侣活性涉及调节各种生物过程，包括神经丝蛋白的磷酸化和轴突运输。HSP27/HSPB1 形成同源寡聚体和同源二聚体，后者在激活后转变为单体状态。此外，它还参与与 HSPB6 的异源低聚物形成。该蛋白质与 α 和 β 微管蛋白相互作用，并与 TGFB1I1、CRYAB、HSPB8 和 HSPBAP1 进行分子关联，从而形成蛋白质-蛋白质相互作用网络，构成其多种细胞功能的基础。

Human

E. coli

C-His

P04792/NP_001531.1 (M1-K205)

3315  [NCBI]

Approximately 28 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM HEPES, 0.1M KCl, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.