2. 重组蛋白
  3. Receptor Proteins
  4. HTR3D 蛋白, Human (Cell-Free, His, SUMO)

HTR3D 蛋白, Human (Cell-Free, His, SUMO)

目录号: HY-P702326
产品使用指南

HTR3D 蛋白作为血清素 (5-羟色胺/5-HT3) 激活的阳离子选择性通道复合物中的亚基发挥作用。激活后，这些复合物会在神经元中引起快速和去极化反应。这强调了 HTR3D 在介导细胞对血清素的反应中的关键作用，有助于形成涉及神经传递和神经元兴奋性的复杂信号通路网络。HTR3D 蛋白, Human (Cell-Free, His, SUMO) 是重组的 HTR3D 蛋白，由 E. coli Cell-free 表达，带有 N-6*His, N-SUMO 标签。HTR3D 蛋白, Human (Cell-Free, His, SUMO) 全长 234 个氨基酸，分子量为 41.5 kDa。

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描述

HTR3D protein functions as a subunit within serotonin (5-hydroxytryptamine/5-HT3)-activated cation-selective channel complexes. Upon activation, these complexes induce rapid and depolarizing responses in neurons. This underscores the crucial role of HTR3D in mediating cellular responses to serotonin, contributing to the intricate network of signaling pathways involved in neurotransmission and neuronal excitability. HTR3D Protein, Human (Cell-Free, His, SUMO) is the recombinant human-derived HTR3D protein, expressed by E. coli Cell-free , with N-6*His, N-SUMO labeled tag. The total length of HTR3D Protein, Human (Cell-Free, His, SUMO) is 234 a.a., with molecular weight of 41.5 kDa.
研究背景

HTR3D 蛋白是形成血清素 (5-羟色胺/5-HT3) 激活的阳离子选择性通道复合物的关键成分。激活后，这些复合物会在神经元中引起快速的去极化反应。这凸显了 HTR3D 在介导细胞对血清素信号传导的反应中的关键作用。作为这些通道复合物的一部分，HTR3D 有助于调节神经元兴奋性，影响受血清素影响的各种生理过程，例如情绪、食欲和认知。
种属

Human
表达系统

E. coli Cell-free
标签

N-6*His;N-SUMO
蛋白编号

Q70Z44 (V221-T454)
基因 ID

200909
蛋白结构
N-term
6*His-SUMO
HTR3D (V221-T454)
Accession # Q70Z44
C-term
同用名
5-hydroxytryptamine receptor 3D; Serotonin receptor 3D
氨基酸序列

VAIRRRCRPSPYVVNFLVPSGILIAIDALSFYLPLESGNCAPFKMTVLLGYSVFLLMMNDLLPATSTSSHASLVAPLALMQTPLPAGVYFALCLSLMVGSLLETIFITHLLHVATTQPLPLPRWLHSLLLHCTGQGRCCPTAPQKGNKGPGLTPTHLPGVKEPEVSAGQMPGPGEAELTGGSEWTRAQREHEAQKQHSVELWVQFSHAMDALLFRLYLLFMASSIITVICLWNT

分子量

41.5 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

HTR3D Protein, Human (Cell-Free, His, SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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