IdeS 蛋白, Streptococcus pyogenes (N-His)

IdeS Protein is a highly specific IgG endopeptidase evolved from Streptococcus pyogenes, which can degrade IgG and participate in the immune response. IdeS Protein inhibits the function of certain neutrophil effectors, namely the production of reactive oxygen species (ROS), independently of IgG endopeptidase activity. IdeS Protein, Streptococcus pyogenes (N-His) is the recombinant IdeS protein, expressed by E. coli , with N-10*His labeled tag. The total length of IdeS Protein, Streptococcus pyogenes (N-His) is 312 a.a., with molecular weight of ~34 kDa.

IdeS 是由化脓性链球菌进化出一种高度特异性的 IgG 内肽酶，它不仅可以降解 IgG，还可以直接或间接地抑制先天免疫反应，从而促进链球菌在炎症环境中的生存。IdeS 被发现与链球菌的 Mac-1 相同，Mac-1 是一种被认为通过抑制 Fc 受体 (CD16) 对 IgG 和/或补体配置的识别来抑制吞噬的蛋白。IdeS/Mac-1可以抑制某些中性粒细胞效应物的功能，即活性氧 (ROS) 的产生，而不依赖于 IgG 内肽酶的活性。

Measured by its ability to cleave human IgG. The DC₅₀ is 49.93 ng, as measured under the described conditions.
The DC₅₀ is defined as the amount of enzyme required to cleave 50% of 1 μg human IgG in 30 minutes at 37 °C. Use of Recombinant S.pyogenes IdeS in the cleavage of other IgGs may require alternative conditions for optimal performance.

Others

E. coli

N-10*His

F8V4V0 (D30-N341)

/

DSFSANQEIRYSEVTPYHVTSVWTKGVTPPAKFTQGEDVFHAPYVANQGWYDITKTFNGKDDLLCGAATAGNMLHWWFDQNKEKIEAYLKKHPDKQKIMFGDQELLDVRKVINTKGDQTNSELFNYFRDKAFPGLSARRIGVMPDLVLDMFINGYYLNVYKTQTTDVNRTYQEKDRRGGIFDAVFTRGDQSKLLTSRHDFKEKNLKEISDLIKKELTEGKALGLSHTYANVRINHVINLWGADFDSNGNLKAIYVTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQDSWNQTN

Approximately 34 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.