IDO 蛋白, Human (His)

IDO proteins catalyze the first step in tryptophan catabolism via the kynurenine pathway, which is critical for immune tolerance and homeostasis maintenance. IDO Protein, Human (His) is the recombinant human-derived IDO protein, expressed by E. coli , with N-6*His labeled tag. The total length of IDO Protein, Human (His) is 403 a.a., with molecular weight of 40-50 kDa.

正如各种研究所记录的那样，IDO 蛋白通过催化犬尿氨酸途径中必需氨基酸色氨酸分解代谢的初始步骤和限速步骤，在细胞过程中发挥着核心作用。除了其酶促功能外，IDO 还参与外周免疫耐受，通过预防因不受控制和过度反应的免疫反应而导致的自身免疫或免疫病理，从而有助于维持体内平衡。IDO 活性导致色氨酸缺乏，抑制 T 淋巴细胞分裂，色氨酸分解代谢物的积累诱导 T 细胞凋亡，同时促进调节性 T 细胞的分化。此外，IDO 还可作为抗肿瘤免疫的抑制剂，并通过剥夺细胞内病原体的色氨酸来限制其生长。此外，IDO 在保护胎儿免受母体免疫排斥方面发挥着至关重要的作用。这些不同的功能强调了 IDO 在协调免疫反应、维持代谢平衡以及促进对自身免疫、癌症和宿主-病原体相互作用具有影响的重要生理过程中的重要性。

Measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine. The specific activity is 664.3519 pmol/min/µg, as measured under the described conditions.

Human

E. coli

N-6*His

P14902 (M1-G403)

3620  [NCBI]

AHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG

Approximately 40-50 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Sodium Acetate, 150 mM NaCl and 20% Glycerol, pH 4.5 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 20% Glycerol.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.