IgE 蛋白, Human (HEK293, His-Avi)

IgE protein is an important immunoglobulin component that participates in the recognition and effector phases of humoral immunity. As a membrane-bound receptor on B lymphocytes, IgE triggers clonal expansion upon antigen binding, leading to plasma cell differentiation. IgE Protein, Human (HEK293, His-Avi) is the recombinant human-derived IgE protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of IgE Protein, Human (HEK293, His-Avi) is 220 a.a., with molecular weight of 30-40 kDa.

IgE 蛋白是免疫球蛋白的重要组成部分，在体液免疫的识别阶段和效应阶段发挥作用。IgE 在识别阶段作为 B 淋巴细胞上的膜结合受体，在特异性抗原结合后触发克隆扩增和分化为免疫球蛋白分泌浆细胞方面发挥着关键作用。在效应器阶段，分泌的 IgE 通过两种 Fc 受体 FCER1A:MS4A2:FCGR1A 和 FCER2 介导免疫反应，这两种受体在抗原交联时启动导致免疫细胞激活的信号通路。IgE 促进针对过敏原的即时超敏反应，并防御蠕虫寄生虫、细菌和毒液毒性。失调会导致有害的过敏反应和过敏反应。IgE 刺激肥大细胞、嗜碱性粒细胞和嗜酸性粒细胞释放炎症介质，有助于组织重塑和针对微生物的细胞毒性。在巨噬细胞上，IgE 诱导细胞内杀死寄生虫并激活抗肿瘤功能，包括抗体依赖性细胞毒性和促炎反应。此外，IgE 在 B 细胞抗原摄取和呈递给 T 细胞中发挥作用。

Immobilized Human IgE, His Tag at 0.5 μg/mL (100μl/well) on the plate. Dose response curve for Anti-IgE Antibody, hFc Tag with the EC₅₀ of ≤7.0 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

P01854 (C209-K428)

3497  [NCBI]

30-40 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20mM PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.