IgE 蛋白, Mouse (HEK293, His)

IgE is an important component of the immunoglobulin chain that coordinates humoral immunity. As a receptor, membrane-bound IgE initiates clonal expansion and B cell differentiation. IgE Protein, Mouse (HEK293, His) is the recombinant mouse-derived IgE protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of IgE Protein, Mouse (HEK293, His) is 331 a.a., with molecular weight (glycosylation form) of 50-60 kDa.

IgE 是免疫球蛋白重链的重要组成部分，在体液免疫的复杂协调中发挥着关键作用。在识别阶段充当受体，膜结合免疫球蛋白在抗原结合后启动克隆扩增和 B 淋巴细胞分化为浆细胞。在效应阶段，分泌的免疫球蛋白有助于消除结合的抗原。抗原结合位点以对特定抗原的显着亲和力为标志，是由一条重链及其相关轻链的可变结构域之间的协作产生的。通过 V-(D)-J 重排和随后的体细胞超突变，可变结构域经历亲和力成熟，增强对特定抗原的特异性。在结构上，每个免疫球蛋白由两条相同的重链和两条相同的轻链组成，通过二硫键连接。重链和轻链的N端可变区构成抗原结合位点，而重链的C端恒定区与免疫受体相互作用，驱动效应器功能。这种动态相互作用定义了 IgE 在协调体液免疫反应中的多功能和精确作用。

Mouse

HEK293

C-6*His

P06336 (V91-S421)

380792  [NCBI]

VRPVNITEPTLELLHSSCDPNAFHSTIQLYCFIYGHILNDVSVSWLMDDREITDTLAQTVLIKEEGKLASTCSKLNITEQQWMSESTFTCKVTSQGVDYLAHTRRCPDHEPRGVITYLIPPSPLDLYQNGAPKLTCLVVDLESEKNVNVTWNQEKKTSVSASQWYTKHHNNATTSITSILPVVAKDWIEGYGYQCIVDHPDFPKPIVRSITKTPGQRSAPEVYVFPPPEEESEDKRTLTCLIQNFFPEDISVQWLGDGKLISNSQHSTTTPLKSNGSNQGFFIFSRLEVAKTLWTQRKQFTCQVIHEALQKPRKLEKTISTSLGNTSLRPS

50-60 kDa (Due to glycosylation)

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.