IL-23 alpha & IL-12 beta Heterodimer 蛋白, Rat (HEK293, His)

The IL-23 alpha protein has cytokine activity and binds to interleukin-23 receptors. It regulates cytokine production, lymphocyte activation, and peptidyl-tyrosine phosphorylation. It influences T cell proliferation and RNA polymerase II transcription. Found in the extracellular space, it is part of the interleukin-23 complex. It is mainly expressed in the thymus, spleen, and other tissues. It is orthologous to the human IL23A gene encoding interleukin 23 subunit alpha. IL-23 alpha & IL-12 beta Heterodimer Protein, Rat (HEK293, His) is a recombinant protein dimer complex containing rat-derived IL-23 alpha & IL-12 beta Heterodimer protein, expressed by HEK293, with C-10*His labeled tag. IL-23 alpha & IL-12 beta Heterodimer Protein, Rat (HEK293, His), has molecular weight of ~23 & 43 & 48 kDa, respectively.

IL-23 α 蛋白预计具有细胞因子活性并有助于白细胞介素 23 受体的结合。据预测，它还参与多种过程，包括细胞因子产生、淋巴细胞活化和肽基酪氨酸磷酸化的正向调节。此外，预计它在 T 细胞增殖的上游和 RNA 聚合酶 II 转录的正向调节中发挥作用。该蛋白质位于细胞外空间，预计也是白细胞介素 23 复合物的组成部分。在表达方面，它表现出对胸腺 (RPKM 15.2)、脾脏 (RPKM 11.9) 和其他九种组织的偏向。它与负责编码白细胞介素 23 亚基 α 的人类 IL23A 基因具有直系同源性。

1.Measured by its ability to induce IL17 secretion by mouse splenocytes and the ED₅₀ is 0.5-3 ng/mL.
2. Measured by its ability to induce IL17 secretion by mouse CTLL-2 cells. The ED₅₀ for this effect is 4.136 ng/mL, corresponding to a specific activity is 2.41×10⁵ U/mg.

• 
  Measured by its ability to induce IL17 secretion by mouse CTLL-2 cells. The ED₅₀ for this effect is 4.136 ng/mL, corresponding to a specific activity is 2.41×10⁵ U/mg.

Rat

HEK293

C-10*His

NP_569094.1 (V22-A196) & EDM04146.1 (M23-S335)

155140  [NCBI]&64546  [NCBI]

IL-23alpha:
VPRSSSPDWAQCQQLSRNLCTLAWSAHTPVGQMDLLREEGEEETKSDVPRIQCGDGCDPQGLKDNSQFCLQRIRQGLVFYKHLLDSDIFTGEPSLLPDSPVDQLHTSLLGLSQLLQPEDHHWETQQMPRLSPSQQWQRSLLRSKILRSLQAFLAIAARVFAHGAATLTEPLVPTA
IL-12beta:
MWELEKDVYVVEVDWRPDAPGETVTLTCDSPEEDDITWTSDQRRGVIGSGKTLTITVREFLDAGQYTCHRGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVHRNTDLKFNIKSSSSSPESRAVTCGAASLSAEKVTLNQRDYEKYSVACQEDVTCPTAEETLPIELVVEAQQQNKYENYSTSFFIRDIIKPDPPKNLQVKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKTKETEEECNQKGAFLVEKTSAEVQCKGANICVQAQDRYYNSSCSKWTCVPCRGRS

Approximately 23 & 43 & 48 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.