IL-33 蛋白, Human (His)

IL-33 protein, as a cytokine, binds to and signals through the IL1RL1/ST2 receptor, thereby activating the NF-kappa-B and MAPK signaling pathways in target cells. It is involved in the maturation of Th2 cells and contributes to the secretion of T helper cell type 2 related cytokines. IL-33 Protein, Human (His) is the recombinant human-derived IL-33 protein, expressed by E. coli , with N-6*His labeled tag. The total length of IL-33 Protein, Human (His) is 159 a.a., with molecular weight of ~21.0 kDa.

IL-33 蛋白作为细胞因子，与 IL1RL1/ST2 受体结合并通过其发出信号，从而激活靶细胞中的 NF-kappa-B 和 MAPK 信号通路。它参与 Th2 细胞的成熟，有助于分泌 T 辅助细胞 2 型相关细胞因子。此外，IL-33 在肥大细胞、嗜碱性粒细胞、嗜酸性粒细胞和自然杀伤细胞的激活中发挥着至关重要的作用。它作为交替激活的巨噬细胞极化的增强剂，作为 Th2 细胞的化学引诱剂，并可能起到“警报器”的作用，在组织损伤期间放大免疫反应。IL-33 可诱导 UCP2 依赖性线粒体快速重新布线，减少活性氧的产生并保持克雷布斯循环的完整性，这对于衣康酸的持续产生以及随后的 GATA3 依赖性炎症消退分化至关重要激活巨噬细胞。在静止内皮细胞中，未切割形式的 IL-33 组成型且大量表达，作为具有转录抑制特性的染色质相关核因子，可能隔离核 NF-kappaB/RELA，从而降低其靶标的表达；这种形式在血管生成或促炎症激活后迅速消失。

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED₅₀ for this effect is 0.4463 ng/mL, corresponding to a specific activity is 2.254×10⁶ units/mg.

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  Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED₅₀ for this effect is 0.4463 ng/mL, corresponding to a specific activity is 2.254×10⁶ units/mg.

Human

E. coli

N-6*His

O95760 (S112-T270)

90865  [NCBI]

SITGISPITEYLASLSTYNDQSITFALEDESYEIYVEDLKKDEKKDKVLLSYYESQHPSNESGDGVDGKMLMVTLSPTKDFWLHANNKEHSVELHKCEKPLPDQAFFVLHNMHSNCVSFECKTDPGVFIGVKDNHLALIKVDSSENLCTENILFKLSET

Approximately 21.0 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS,1 mM DTT, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.