Integrin beta-1/CD29 蛋白, Human (HEK293, His)

Integrin beta-1/CD29 forms distinct heterodimers that act as multifunctional receptors for extracellular matrix proteins. It recognizes collagen, fibronectin, fibrinogen and laminin and plays a key role in cell functions such as migration, adhesion and angiogenesis. Integrin beta-1/CD29 Protein, Human (708a.a, HEK293, His) is the recombinant human-derived Integrin beta-1/CD29 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Integrin beta-1/CD29 Protein, Human (708a.a, HEK293, His) is 708 a.a., with molecular weight of 100-130 kDa.

整合素 beta-1/CD29 蛋白与各种 α 亚基形成异二聚体，可作为多种细胞外基质蛋白的多功能受体。整合素 α-1/β-1、α-2/β-1、α-10/β-1 和 α-11/β-1 识别胶原蛋白，而整合素 α-2/β-2、α-3/ beta-1、alpha-4/beta-1、alpha-5/beta-1、alpha-8/beta-1、alpha-10/beta-1、alpha-11/beta-1 和 alpha-V/beta -1 作为纤连蛋白受体。此外，整合素 α-5/β-1 作为纤维蛋白原的受体，整合素 α-1/β-1、α-2/β-1、α-6/β-1 和 α-7/β- 1 是层粘连蛋白的受体。特定的整合素，例如 α-4/β-1 和 α-9/β-1，在识别 VCAM1 和其他配体中发挥作用，有助于多种细胞功能，包括迁移、粘附和血管生成。Beta-1 整合素可识别 RGD 序列，参与多种生理过程，例如成骨细胞压缩、细胞运动和胞质分裂完成。此外，整合素 beta-1/CD29 在促进细胞侵袭、基质降解和成肌细胞分化中发挥着关键作用，凸显了其在细胞粘附、信号传导和发育过程中的重要性。

Measured by its binding ability in a functional ELISA. Immobilized human Fibronectin, at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD29 protein. The ED₅₀ for this effect is 306.6 ng/mL.

Human

HEK293

C-6*His

P05556 (Q21-D728)

3688  [NCBI]

QTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPD

100-145 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.