ITK 蛋白, Mouse (sf9, His-GST)

ITK protein is a tyrosine kinase that plays a crucial role in directing adaptive immune responses by controlling the development, function, and differentiation of conventional T cells and unconventional NKT cells. After T cell receptor (TCR) activation by antigen-presenting cells (APC), ITK is recruited to the cell membrane and phosphorylated by LCK, thereby making it fully activated. ITK Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived ITK protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of ITK Protein, Mouse (sf9, His-GST) is 269 a.a., with molecular weight of ~58 kDa.

ITK 蛋白是一种酪氨酸激酶，通过控制传统 T 细胞和非常规 NKT 细胞的发育、功能和分化，在协调适应性免疫反应中发挥着关键作用。抗原呈递细胞 (APC) 激活 T 细胞受体 (TCR) 后，ITK 被募集到细胞膜上，靠近受刺激的 TCR 受体，并在其中被 LCK 磷酸化。这种磷酸化会引发 ITK 自磷酸化，最终导致其完全激活。一旦被激活，ITK 就会磷酸化 PLCG1，从而激活该脂肪酶并触发其底物的后续裂解。因此，钙从内质网释放到细胞质中，促进活化 T 细胞核激活剂 (NFAT) 易位到细胞核中以执行其转录功能。ITK 还会磷酸化两种必需的衔接蛋白，即用于激活 T 细胞/LAT 蛋白和 LCP2 的连接蛋白，从而导致各种信号分子（例如 VAV1）的募集。这种复杂的信号级联最终导致淋巴因子的产生、T 细胞增殖和分化。此外，ITK对于γ-δ T细胞中TCR介导的钙反应是不可或缺的，可能影响未成熟γ-δ T细胞的Vgamma2阳性亚群中的转录组特征。此外，ITK 在“Tyr-525”处磷酸化 TBX21，介导其与 GATA3 的相互作用。ITK 的多方面功能强调了其在塑造适应性免疫反应中的关键作用。

The specific activity of ITK was determined to be 54.33 nmol/min/mg using a myelin basic protein (MBP) substrate.

Mouse

Sf9 insect cells

N-His;N-GST

Q03526 (R351-L619)

16428  [NCBI]

Approximately 58 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.5, 10% Glycerol, 0.5 mM GSH. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.