1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Kallikrein-3/PSA 蛋白, Human (237a.a, HEK293, His)

Kallikrein-3/PSA 蛋白, Human (237a.a, HEK293, His)

目录号: HY-P70606
COA 产品使用指南

激肽释放酶-3 (PSA) 蛋白在男性生殖系统中至关重要,可水解精囊蛋白 Semenogelin-1。这种酶活性引发精液凝块液化,这对于精子的活动性和生育能力至关重要。PSA 对 Semenogelin-1 的裂解凸显了其在精液生理学和男性生殖功能的复杂过程中的重要性。Kallikrein-3/PSA 蛋白, Human (237a.a, HEK293, His) 是重组的 Kallikrein-3/PSA 蛋白,由 HEK293 表达,带有 C-6*His 标签。Kallikrein-3/PSA 蛋白, Human (237a.a, HEK293, His) 全长 237 个氨基酸,分子量约为 ~35.0 kDa。

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规格 价格 是否有货 数量
10 μg ¥1600 In-stock
50 μg ¥4500 In-stock
100 μg   询价  

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  • 生物活性

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描述

Kallikrein-3 (PSA) protein, pivotal in the male reproductive system, hydrolyzes semenogelin-1, a seminal vesicle protein. This enzymatic activity initiates seminal coagulum liquefaction, crucial for sperm mobility and fertility. PSA's cleavage of semenogelin-1 highlights its importance in the complex processes of semen physiology and male reproductive function. Kallikrein-3/PSA Protein, Human (237a.a, HEK293, His) is the recombinant human-derived Kallikrein-3/PSA protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Kallikrein-3/PSA Protein, Human (237a.a, HEK293, His) is 237 a.a., with molecular weight of ~35.0 kDa.

研究背景

激肽释放酶-3 (PSA) 蛋白通过水解精囊蛋白 Semenogelin-1,在男性生殖系统中发挥关键作用。这种酶活性负责启动精浆的液化,这是精子活动性和整体生育力的关键步骤。PSA 裂解 Semenogelin-1 的能力强调了其在涉及精液生理学和男性生殖功能的复杂过程中的重要性。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P07288(I25-P261)

基因 ID

354  [NCBI]

蛋白结构
N-term
PSA (I25-P261)
Accession # P07288
6*His
C-term
同用名
Prostate-specific antigen; PSA; Gamma-seminoprotein; Kallikrein-3; P-30 antigen; Semenogelase; KLK3; APS
氨基酸序列

IVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP

分子量

Approximately 35.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl,10 mM CaCl2, 0.05% Brij 35, pH 7.5.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Kallikrein-3/PSA Protein, Human (237a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Kallikrein-3/PSA Protein, Human (237a.a, HEK293, His)
目录号:
HY-P70606
需求量: