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  4. KCNK13 蛋白, Human (Cell-Free, His)

KCNK13 蛋白, Human (Cell-Free, His)

目录号: HY-P702347
产品使用指南

KCNK13 蛋白是一种钾通道，在对称 K(+) 溶液中具有独特的弱内向整流功能。作为同二聚体，它调节钾离子穿过细胞膜的流动，影响细胞兴奋性和膜电位。这一特性凸显了 KCNK13 在塑造电化学梯度、影响依赖钾离子动力学的过程中的作用。KCNK13 蛋白, Human (Cell-Free, His) 是重组的 KCNK13 蛋白，由 E. coli Cell-free 表达，带有 N-10*His 标签。KCNK13 蛋白, Human (Cell-Free, His) 全长 408 个氨基酸，分子量为 51.4 kDa。

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描述

KCNK13 Protein, a potassium channel, uniquely features weak inward rectification in symmetrical K(+) solution. As a homodimer, it regulates potassium ion flow across cellular membranes, impacting cellular excitability and membrane potential. This property highlights KCNK13's role in shaping electrochemical gradients, influencing processes reliant on potassium ion dynamics. KCNK13 Protein, Human (Cell-Free, His) is the recombinant human-derived KCNK13 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of KCNK13 Protein, Human (Cell-Free, His) is 408 a.a., with molecular weight of 51.4 kDa.
研究背景

KCNK13 蛋白是一种钾通道，在对称 K(+) 溶液中表现出独特的功能特征，其特征是弱向内整流。作为同型二聚体，KCNK13 有助于调节钾离子穿过细胞膜的流动，在细胞兴奋性和膜电位的调节中发挥作用。这种弱内向整流的独特特性强调了 KCNK13 参与塑造跨细胞膜的电化学梯度，最终影响依赖钾离子动力学的细胞过程。
种属

Human
表达系统

E. coli Cell-free
标签

N-10*His
蛋白编号

Q9HB14 (M1-R408)
基因 ID

56659
蛋白结构
N-term
10*His
KCNK13 (M1-R408)
Accession # Q9HB14
C-term
同用名
Potassium channel subfamily K member 13; Tandem pore domain halothane-inhibited potassium channel 1; THIK-1
氨基酸序列

MAGRGFSWGPGHLNEDNARFLLLAALIVLYLLGGAAVFSALELAHERQAKQRWEERLANFSRGHNLSRDELRGFLRHYEEATRAGIRVDNVRPRWDFTGAFYFVGTVVSTIGFGMTTPATVGGKIFLIFYGLVGCSSTILFFNLFLERLITIIAYIMKSCHQRQLRRRGALPQESLKDAGQCEVDSLAGWKPSVYYVMLILCTASILISCCASAMYTPIEGWSYFDSLYFCFVAFSTIGFGDLVSSQNAHYESQGLYRFANFVFILMGVCCIYSLFNVISILIKQSLNWILRKMDSGCCPQCQRGLLRSRRNVVMPGSVRNRCNISIETDGVAESDTDGRRLSGEMISMKDLLAANKASLAILQKQLSEMANGCPHQTSTLARDNEFSGGVGAFAIMNNRLAETSGDR

分子量

51.4 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

KCNK13 Protein, Human (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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