1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. KDM2A 蛋白, Human

KDM2A 蛋白, Human

目录号: HY-P701621
产品使用指南

KDM2A 蛋白是一种针对组蛋白 H3 的“Lys-36”的组蛋白去甲基化酶。它在组蛋白密码中起着关键作用,尤其是二甲基化 H3“Lys-36”的去甲基化。甲基化。除了组蛋白去甲基化之外,KDM2A 还识别并结合磷酸化蛋白质,促进其泛素化和降解。KDM2A 蛋白, Human 是重组的 KDM2A 蛋白,由 E. coli 表达,不带标签。KDM2A 蛋白, Human 全长 115 个氨基酸。

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规格 是否有货
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描述

The KDM2A protein is a histone demethylase targeting "Lys-36" of histone H3. It plays a key role in the histone code, especially the demethylation of dimethylated H3 "Lys-36". Methylation. In addition to histone demethylation, KDM2A also recognizes and binds phosphorylated proteins, promoting their ubiquitination and degradation. KDM2A Protein, Human is the recombinant human-derived KDM2A protein, expressed by E. coli , with tag free. The total length of KDM2A Protein, Human is 115 a.a., .

研究背景

KDM2A 蛋白是一种组蛋白去甲基化酶,通过特异性靶向组蛋白 H3 的“Lys-36”,在组蛋白密码中发挥关键作用。该酶表现出对二甲基化 H3 'Lys-36' 残基去甲基化的偏好,对单甲基化和三甲基化 H3 'Lys-36' 表现出弱活性或无活性。除了其组蛋白去甲基化酶功能之外,KDM2A 通过识别和结合某些磷酸化蛋白质、促进其泛素化和随后的降解而展现出多功能性。KDM2A 与着丝粒相关,对于维持异染色质状态至关重要,它抑制卫星重复簇编码的小非编码 RNA 的转录,这对于着丝粒完整性和基因组稳定性至关重要,尤其是在有丝分裂期间。此外,KDM2A 通过抑制 CLOCK-BMAL1 异二聚体和 RORA 的转录激活剂活性来影响昼夜节律基因表达,证明在昼夜节律中具有催化独立的调节作用。KDM2A 的多方面功能强调了它在染色质动力学和细胞过程的复杂协调中的重要性。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

Q9Y2K7 (R567-S681)

基因 ID

22992

蛋白结构
N-term
KDM2A (R567-S681)
Accession # Q9Y2K7
C-term
同用名
KDM2A; Lysine-specific demethylase 2A; CXXC-type zinc finger protein 8; F-box and leucine-rich repeat protein 11; F-box protein FBL7; F-box protein Lilina; F-box/LRR-repeat protein 11; JmjC domain-containing histone demethylation protein 1A; [Histone-H3]-lysine-36 demethylase 1A
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

KDM2A Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HY-P701621
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