Kallikrein-7 蛋白, Human (HEK293, His)

Kallikrein-7 is a serine protease with chymotrypsin-like activity that plays a role in intercellular proteolysis, leading to epidermal exfoliation. It is involved in cancer invasion and metastasis, and elevated expression is associated with poor prognosis and cancer progression. Kallikrein-7 Protein, Human (HEK293, His) is the recombinant human-derived Kallikrein-7 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Kallikrein-7 Protein, Human (HEK293, His) is 231 a.a., with molecular weight of 30-33 KDa.

激肽释放酶-7 是丝氨酸蛋白酶激肽释放酶亚家族的成员，表现出胰凝乳蛋白酶样活性，并参与脱皮（最外表皮层脱落）之前细胞间粘附结构的蛋白水解。这种酶的多种生理功能延伸到其参与癌症相关过程，包括侵袭和转移。Kallikrein-7 基因的表达升高与各种癌症类型的不良预后和进展相关。该基因的多态性可能导致特应性皮炎的发生。该基因位于 19 号染色体上的基因簇内，具有多个选择性剪接的转录变体，反映了其复杂性并参与各种细胞过程。由于在皮肤 (RPKM 68.1) 和食道 (RPKM 25.3) 中观察到偏向表达，Kallikrein-7 作为一种多面蛋白酶出现，对正常生理和疾病状态都有影响。

Measured by its ability to cleave the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK (Dnp) NH2 and the specific activity is >150 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

Human

HEK293

C-10*His

NP_005037.1 (E23-R253)

5650  [NCBI]

EEAQGDKIIDGAPCARGSHPWQVALLSGNQLHCGGVLVNERWVLTAAHCKMNEYTVHLGSDTLGDRRAQRIKASKSFRHPGYSTQTHVNDLMLVKLNSQARLSSMVKKVRLPSRCEPPGTTCTVSGWGTTTSPDVTFPSDLMCVDVKLISPQDCTKVYKDLLENSMLCAGIPDSKKNACNGDSGGPLVCRGTLQGLVSWGTFPCGQPNDPGVYTQVCKFTKWINDTMKKHR

30-33 kDa.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.