Ku70-Ku80 Heterodimer 蛋白, Human (sf9, His)

The Ku70, a single-stranded DNA-dependent ATP-dependent helicase, is pivotal in DNA non-homologous end joining (NHEJ), central to double-strand break repair and V(D)J recombination. Operating as a DNA helicase II complex with a cell cycle-dependent preference for double-stranded DNA fork-like ends in the 3'-5' direction, it recognizes and binds to broken DNA ends during NHEJ, protecting them from resection. As a DNA-PK regulatory subunit, it enhances PRKDC's affinity to DNA, stabilizes broken DNA ends, and facilitates ligation in NHEJ. The heterodimer may act as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase) near double-strand breaks. Beyond DNA repair, it regulates transcription, osteocalcin expression, early ribosome assembly steps, and forms complexes involved in the innate immune response, showcasing its multifaceted cellular functions. Ku70-Ku80 Heterodimer Protein, Human (sf9, His) is a recombinant protein dimer complex containing human-derived Ku70-Ku80 Heterodimer protein, expressed by Sf9 insect cells, with N-His labeled tag. Ku70-Ku80 Heterodimer Protein, Human (sf9, His), has molecular weight of ~70 & 85 kDa, respectively.

Ku70 是一种单链 DNA 依赖性 ATP 依赖性解旋酶，对于 DNA 非同源末端连接 (NHEJ) 至关重要，在双链断裂修复和 V(D)J 重组中发挥核心作用。作为 DNA 解旋酶 II 复合物，它对双链 DNA 的叉状末端表现出细胞周期依赖性偏好，并在 3'-5' 方向上发挥作用。在 NHEJ 过程中，异二聚体识别并结合断裂的 DNA 末端，保护它们免遭进一步切除。它作为 DNA 依赖性蛋白激酶复合物 (DNA-PK) 的调节亚基，显着增强催化亚基 PRKDC 对 DNA 的亲和力。Ku70 参与稳定断裂的 DNA 末端并将它们聚集在一起以进行 NHEJ 中的后续连接步骤。此外，它还可作为 5'-脱氧核糖-5-磷酸裂解酶 (5'-dRP 裂解酶)，促进消除双链断裂附近的脱碱基位点的 5'-脱氧核糖-5-磷酸。除了在 DNA 修复中的作用外，异二聚体还参与转录、骨钙素表达和核糖体组装的早期步骤的调节。它还形成参与先天免疫反应的复合物，并与各种相互作用的伙伴相关联，突出其在细胞过程中的多方面功能。

Human

Sf9 insect cells

N-His

P13010 (M1-I732)&P12956 (M1-D609)

7520  [NCBI]&2547  [NCBI]

Approximately 70&85 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.